The Drosophila female sterile mutation twin peaks is a novel allele of tramtrack and reveals a requirement for Ttk69 in epithelial morphogenesis
The Drosophila female sterile mutation twin peaks is a novel allele of tramtrack and reveals a requirement for Ttk69 in epithelial morphogenesis
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DOI：
10.1006/dbio.2002.0856
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发表时间：
2003-01-01
影响因子：
2.7
通讯作者：
Berg, CA
中科院分区：
文献类型：
--
作者：
French, RL;Cosand, KA;Berg, CA
果蝇基因tramtrack（ttk）编码两个转录抑制子，Ttk69和Ttk88，这是正常胚胎发生和成虫盘发育所必需的。在这里，我们描述了一种新的女性不育的tramtrack等位基因称为双峰（ttk（twk）），不像其他tramtrack等位基因，没有影响生存能力，并产生没有明显的形态缺陷，除了在卵子发生。双峰纯合子的雌性产的蛋很小，蛋壳薄，背部有短的呼吸附属物。互补分析，免疫定位，和救援数据表明，这些缺陷是由于Ttk69的损失，这是在卵泡细胞中表达，并需要正常的绒毛膜生产和背侧卵泡细胞迁移。对调控蛋壳合成的其他基因座突变产生的表型分析表明，绒毛膜生成和卵泡细胞迁移缺陷是独立的。我们提出的证据表明，双峰破坏了Ttk69的后期卵泡细胞表达所需的启动子或启动子。我们推测，Ttk69在所有的卵泡细胞中的损失破坏绒毛膜基因的表达和缺乏功能的背侧前卵泡细胞抑制形态发生的变化所需的延长背侧附属物。(C)2003年爱思唯尔科学（美国）。
The Drosophila gene tramtrack (ttk) encodes two transcriptional repressors, Ttk69 and Ttk88, which are required for normal embryogenesis and imaginal disc development. Here, we characterize a novel female sterile allele of tramtrack called twin peaks (ttk(twk)) that, unlike other tramtrack alleles, has no effect on viability and produces no obvious morphological defects, except during oogenesis. Females homozygous for twin peaks produce small eggs with thin eggshells and short dorsal respiratory appendages. Complementation analyses, immunolocalization, and rescue data demonstrate that these defects are due to loss of Ttk69, which is expressed in the follicle cells and is required for normal chorion production and dorsal follicle-cell migration. Analyses of phenotypes produced by mutations in other loci that regulate eggshell synthesis suggest that the chorion production and follicle-cell migration defects are independent. We present evidence that twin peaks disrupts a promoter or promoters required for late-stage follicle-cell expression of Ttk69. We hypothesize that loss of Ttk69 in all follicle cells disrupts chorion gene expression and lack of function in dorsal anterior follicle cells inhibits morphogenetic changes required for elongating the dorsal appendages. (C) 2003 Elsevier science (USA).