Type 2 iodothyronine deiodinase is highly expressed in medullary thyroid carcinoma.
Type 2 iodothyronine deiodinase is highly expressed in medullary thyroid carcinoma.
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2 型碘甲状腺原氨酸脱碘酶在甲状腺髓样癌中高表达。
DOI：
10.1016/j.mce.2008.04.009
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发表时间：
2008
影响因子：
4.1
通讯作者：
Maia,AnaLuiza
中科院分区：
文献类型：
--
作者：
Meyer,ErikaLSouza;Goemann,IuriM;Dora,JoséMiguel;Wagner,MarciaS;Maia,AnaLuiza
II型脱碘酶（D2）在控制细胞内T3浓度中起关键作用，早期研究表明，甲状腺中D2活性起源于滤泡而非滤泡旁的c细胞。在这里，我们发现D2在人甲状腺髓样癌（MTC）中高度表达，这是一种由c细胞引起的肿瘤。在12例未选择的MTC患者的所有MTC样本中检测到D2转录本，D2活性水平与周围正常滤泡组织的水平相当（0.41±0.10fmolminmgprotein vs. 0.43±0.41fmolminmgprotein， P=0.91）。对人MTC细胞系TT细胞的进一步分析表明，甲状腺激素下调D2的表达，cAMP类似物和地塞米松增强D2的表达。在所有MTC样本和TT细胞中也检测到甲状腺激素受体α1和β亚型，从而提示D2在肿瘤组织中局部产生T3的潜在作用。
Type II deiodinase (D2) plays a critical role in controlling intracellular T3 concentration and early studies indicated a follicular but not a parafollicular C-cell origin of D2 activity in the thyroid gland. Here, we show that D2 is highly expressed in human medullary thyroid carcinoma (MTC), a tumor that arises from the C-cells. D2 transcripts were detected in all MTC samples obtained from 12 unselected MTC patients and the levels of D2 activity were comparable to those found in surrounding normal follicular tissue (0.41±0.10fmolminmgprotein vs. 0.43±0.41fmolminmgprotein, P=0.91). Additional analysis in the TT cells, a human MTC cell line, demonstrated that the D2 expression is downregulated by thyroid hormones and enhanced by cAMP analogs and dexamethasone. The thyroid hormone receptor α1 and β isoforms were also detected in all MTC samples and in TT cells, thus suggesting a potential role of T3 locally produced by D2 in this neoplastic tissue.