Isolation and characterization of a novel single-stranded RNA virus infectious to a marine fungoid protist, Schizochytrium sp (Thraustochytriaceae, labyrinthulea)
Isolation and characterization of a novel single-stranded RNA virus infectious to a marine fungoid protist, Schizochytrium sp (Thraustochytriaceae, labyrinthulea)
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DOI：
10.1128/aem.71.8.4516-4522.2005
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发表时间：
2005-08-01
影响因子：
4.4
通讯作者：
Honda, D
中科院分区：
文献类型：
--
作者：
Takao, Y;Nagasaki, K;Honda, D
破囊壶菌是世界性渗透异养微生物，作为分解者、多不饱和脂肪酸生产者和软体动物病原体发挥着重要作用，特别是在沿海生态系统中。 SssRNAV，一种新型单链 RNA (ssRNA) 病毒，感染海洋类真菌原生生物裂殖壶菌 (Schizochytrium sp.)。 （Labyrinthulea，破囊壶菌科）于2000年7月从日本神户港近海中分离出来，并对其基本特征进行了检查。病毒颗粒呈二十面体，无尾部，长约。直径25纳米。 SssRNAV在宿主细胞的细胞质内形成晶体阵列和随机组装，并且也沿着细胞内膜结构集中。通过一步生长实验，裂解周期和爆发大小估计为
Thraustochytrids are cosmopolitan osmoheterotrophic microorganisms that play important roles as decomposers, producers of polyunsaturated fatty acids, and pathogens of mollusks, especially in coastal ecosystems. SssRNAV, a novel single-stranded RNA (ssRNA) virus infecting the marine fungoid protist Schizochytrium sp. (Labyrinthulea, Thraustochytriaceae) was isolated from the coastal water of Kobe Harbor, Japan, in July 2000, and its basic characteristics were examined. The virus particle is icosahedral, lacks a tail, and is ca. 25 nm in diameter. SssRNAV formed crystalline arrays and random assemblies within the cytoplasm of host cells, and it was also concentrated along the intracellular membrane structures. By means of one-step growth experiments, the lytic cycle and the burst size were estimated to be