A comparative study of the distribution of alpha- and gamma-enolase subunits in cultured rat neural cells and fibroblasts
A comparative study of the distribution of alpha- and gamma-enolase subunits in cultured rat neural cells and fibroblasts
复制标题
DOI：
10.1016/s0736-5748(96)00090-1
复制
发表时间：
1997-04-01
影响因子：
1.8
通讯作者：
Sensenbrenner, M
中科院分区：
文献类型：
--
作者：
Deloulme, JC;Helies, A;Sensenbrenner, M
我们报道了二聚体糖酵解烯醇化酶（2-磷酸- d -甘油水解酶）在培养的神经细胞中的α（普遍存在）和γ（神经元特异性）亚基的存在和分布。体内仅在神经元和神经内分泌细胞中发现高水平的gy烯醇化酶。培养的神经细胞也含有较高水平的α - γ和α - γ烯醇化酶。在这里，我们通过酶和免疫学技术表明，γ亚基也在培养的大鼠星形胶质细胞和脑膜成纤维细胞中表达，正如我们之前报道的那样，在少突胶质细胞中表达。神经元特异性亚型α γ和γ γ均在所有这些细胞中表达，但α α亚型占总烯醇化酶活性的主要部分。α γ和γ γ烯醇化酶活性的总和随着培养时间的增加而增加，即成熟过程，在少突胶质细胞中达到最高水平（占总烯醇化酶活性的40%），在星形胶质细胞和成纤维细胞中分别达到总酶活性的15%和10%。γ烯醇化酶转录物不仅存在于培养的神经细胞中，也存在于培养的少突胶质细胞、星形胶质细胞和脑膜成纤维细胞中。我们的数据表明，神经元特异性烯醇化酶作为神经元细胞分化的特异性标记物应谨慎使用。(c) 1997 isdn。
We report the presence and distribution of alpha (ubiquitous) and gamma (neuron-specific) subunits of the dimeric glycolytic enzyme enolase (2-phospho-D-glycerate hydrolase) in cultured neural cells. The gy enolase is found in vivo at high levels only in neurons and neuroendocrine cells. Neuronal cells in culture also contain relatively high levels of alpha gamma and gamma gamma enolase. Here we show, by enzymatic and immunological techniques, that the gamma subunit also is expressed in cultured rat astrocytes and meningeal fibroblasts and, as we previously reported, in oligodendrocytes. Both neuron-specific isoforms alpha gamma and gamma gamma are expressed in all these cells, but the alpha alpha isoform accounts for the major part of total enolase activity. The sum of alpha gamma and gamma gamma enolase activities increases with time in culture, i.e. maturation processes, reaching the highest level in oligodendroytes (40% of total enolase activity) and 15 and 10% of total enzymatic activity in astrocytes and fibroblasts, respectively. The gamma enolase transcripts were found not only in cultured neuronal cells but also in cultured oligodendrocytes, astrocytes, and meningeal fibroblasts. Our data indicate that neuron-specific enolase should be used with caution as a specific marker for neuronal cell differentiation. (C) 1997 ISDN.