Epigenetic regulation of mammalian pericentric heterochromatin in vivo by HP1
Epigenetic regulation of mammalian pericentric heterochromatin in vivo by HP1
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DOI：
10.1016/j.bbrc.2005.09.132
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发表时间：
2005-11-25
影响因子：
3.1
通讯作者：
Brown, JP
中科院分区：
文献类型：
--
作者：
Kourmouli, N;Sun, YM;Brown, JP
我们开发了一个模型系统，在缺乏SUV(3)9H1/2组蛋白甲基转移酶(HMTase)活性的ES细胞中，HP1可以靶向着丝粒周围异染色质。如此靶向的HP1可以在着丝点周围异染色质重构组蛋白H3的三甲基化赖氨酸9(Me(3)K9143)和组蛋白H4的三甲基化赖氨酸20(Me(3)K20H4)，表明HP1可以调节这些组蛋白修饰在体内的分布。HP1亚型之间的同型和异型相互作用以及HP1与ESET/SETDB1 HMTase和ATRX染色质重塑酶之间的相互作用在体内都得到了证实。我们得出结论，HP1不仅能“破译”组蛋白密码，而且还能“编码”它。(C)2005 Elsevier Inc.保留所有权利。
We developed a model system whereby HP1 can be targeted to pericentric heterochromatin in ES cells lacking Suv(3)9h1/2 histone methyltransferase (HMTase) activities. HP1 so targeted can reconstitute tri-methylated lysine 9 of histone H3 (Me(3)K9143) and tri-methylated lysine 20 of histone H4 (Me(3)K20H4) at pericentric heterochromatin, indicating that HP1 can regulate the distribution of these histone modifications in vivo. Both homo- and hetero-typic interactions between the HP1 isotypes were demonstrated in vivo as were HP1 interactions with the ESET/SETDB1 HMTase and the ATRX chromatin remodelling enzyme. We conclude that HP1 not only "deciphers" the histone code but can also "encode it." (c) 2005 Elsevier Inc. All rights reserved.