REDUCED VIRULENCE OF SERPULINA-HYODYSENTERIAE HEMOLYSIN-NEGATIVE MUTANTS IN PIGS AND THEIR POTENTIAL TO PROTECT PIGS AGAINST CHALLENGE WITH A VIRULENT-STRAIN
REDUCED VIRULENCE OF SERPULINA-HYODYSENTERIAE HEMOLYSIN-NEGATIVE MUTANTS IN PIGS AND THEIR POTENTIAL TO PROTECT PIGS AGAINST CHALLENGE WITH A VIRULENT-STRAIN
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DOI：
10.1128/iai.62.6.2244-2248.1994
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发表时间：
1994-06-01
影响因子：
3.1
通讯作者：
JOENS, LA
中科院分区：
文献类型：
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作者：
HYATT, DR;TERHUURNE, AAHM;JOENS, LA
研究了由tlyA基因编码的水痢瑟螺旋体溶血素在猪痢疾发病机制中的作用。对两株水痢链球菌（B204和C5）的tlyA突变体进行了猪毒力测定。没有一只动物患上SD。然而，感染野生型菌株B204和C5后，SD的发生率分别为100%和60%。因此，水痢链球菌的tlya编码溶血素是SD的重要毒力因子。研究人员还研究了这些突变体保护猪免受致命的水痢疾链球菌菌株攻击的潜力。用野生型菌株B204攻毒后，先前接种过B204 tlyA突变体的猪中有50%得到保护，而所有对照猪都感染了SD。先前接种C5 tlyA突变体的猪在野生型菌株B204攻毒后没有发生SD，而在本实验中，40%的对照猪发生了SD。因此，先前在猪体内定植的水痢疾葡萄球菌tlyA突变体提供了部分保护，以抵御毒力强的水痢疾葡萄球菌菌株的攻击。
The role of the Serpulina hyodysenteriae hemolysin encoded by the tlyA gene in the pathogenesis of swine dysentery (SD) was studied. tlyA mutants of two S. hyodysenteriae strains (B204 and C5) were tested for virulence in pigs. None of the animals developed SD. Hoc-ever, after infection with wild-type strain B204 or C5, the incidence of SD was 100 or 60%, respectively. Thus, the tlyA-encoded hemolysin of S. hyodysenteriae is an important virulence factor in SD. The potential of these mutants to protect pigs against challenge with a virulent S. hyodysenteriae strain was also studied. After challenge with wild-type strain B204, 50% of pigs previously inoculated with the B204 tlyA mutant were protected, whereas all control pigs contracted SD. None of the pigs previously inoculated,vith the C5 tlyA mutant developed SD upon challenge with wild-type strain B204, whereas 40% of the control pigs developed SD in this experiment. Thus, previous colonization with S. hyodysenteriae tlyA mutants in pigs provides partial protection against challenge with a virulent S. hyodysenteriae strain.