Regulation of the cell cycle by B-Myb
Regulation of the cell cycle by B-Myb
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DOI：
10.1006/bcmd.2001.0399
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发表时间：
2001-03-01
影响因子：
2.3
通讯作者：
Watson, RJ
中科院分区：
文献类型：
--
作者：
Bessa, M;Joaquin, M;Watson, RJ
B-Myb是Myb转录因子的细胞周期调节成员，并且与c-Myb一样，参与造血细胞增殖和分化的调节。在这项研究中，我们研究了B-Myb调节细胞周期的机制。我们发现B-Myb促进Saos-2细胞进入细胞周期S期和克服视网膜母细胞瘤相关p107蛋白过表达介导的G1期阻滞的能力与B-Myb与p107形成体内复合物的能力相关，但与其反式激活功能无关。使用B-Myb显性阴性蛋白的进一步实验表明，通过Myb DNA结合序列调节的基因的转录激活是细胞增殖所必需的。因此，我们的实验表明，B-Myb在两个不同的水平上影响细胞周期进程：通过抑制p107和诱导特定靶基因的转录。(C)北京：科学出版社.
B-Myb is a cell-cycle-regulated member of the Myb transcription factor and, like c-Myb, has been implicated in regulation of hematopoietic cell proliferation and differentiation. In this study we have examined the mechanisms by which B-Myb regulates the cell cycle. We found that the ability of B-Myb both to promote Saos-2 cells into the S phase of the cell cycle and to overcome G1 arrest mediated by overexpression of the retinoblastoma-related p107 protein was correlated with the capacity of B-Myb to form an in vivo complex with p107, but was independent of its transactivation function. Further experiments using a B-Myb dominant-negative protein suggested that transcriptional activation of genes regulated through Myb DNA-binding sequences was required for cell proliferation. Our experiments suggest, therefore, that B-Myb influences cell cycle progression at two distinct levels: by inhibiting p107 and by inducing transcription of specific target genes. (C) 2001 Academic Press.