Endogenous murine leukemia virus-encoded proteins in radiation leukemias of BALB/c mice.
Endogenous murine leukemia virus-encoded proteins in radiation leukemias of BALB/c mice.
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BALB/c 小鼠放射性白血病中的内源性鼠白血病病毒编码蛋白。
DOI：
10.1016/0042-6822(82)90520-7
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发表时间：
1982
期刊：
影响因子：
3.7
通讯作者：
Fleissner,E
中科院分区：
文献类型：
--
作者：
Tress,E;Pierotti,M;DeLeo,AB;O'Donnell,PV;Fleissner,E
为了探讨内源性逆转录病毒在辐射诱导的小鼠白血病发生中的作用，我们用脉冲追逐标记技术和单抗和单抗的血清学分型方法检测了9种BALB/c白血病的病毒编码蛋白。在所有病例中都观察到了主要的GAG前体蛋白Pr65Gag，但只有3例白血病表达了可检测到的糖基化GAG种类gP95Gag或其前体Pr75Gag。没有发现合成Gag-宿主融合蛋白的证据。所有的白血病都没有释放传染性的异嗜性或双嗜性病毒，但所有9例白血病都至少表达了一种具有异质性的包膜蛋白。在两个实例中，针对MuLV G IX抗原的单抗35 56与这类包膜蛋白显示出独特的反应活性，尽管该抗体与复制的异嗜性病毒不起反应。从BALB/c放射线白血病分离株分离的强致白血病病毒诱导的白血病中，观察到一种表型与35.56表型相同的向异/向同向重组env蛋白。
To explore the role of endogenous retroviruses in radiation-induced leukemogenesis in the mouse, we have examined virus-encoded proteins in nine BALB/c leukemias by pulse-chase labeling procedures and serological typing with monospecific and monoclonal antibodies. The major gag precursor protein, Pr65 gag, was observed in all cases, but only three leukemias expressed detectable amounts of the glycosylated gag species, gP95 gag, or its precursor, Pr75 gag. No evidence was found for synthesis of gag-host fusion proteins. None of the leukemias released infectious xenotropic or dualtropic virus, but all nine expressed at least one env protein with xenotropic properties. In two instances a monoclonal antibody, 35 56, which is specific for the MuLV G IX antigen, displayed a distinctive reactivity with this class of env protein, although this antibody is unreactive with replicating xenotropic viruses. An ecotropic/xenotropic recombinant env protein with the same 35 56 phenotype was observed in a leukemia induced by a strongly leukemogenic virus isolated from a BALB/c radiation leukemia.