Effects of Chinese herbal medicine fuzhisan on aged rats
Effects of Chinese herbal medicine fuzhisan on aged rats
复制标题
DOI：
10.1016/j.exger.2008.05.018
复制
发表时间：
2008-09-01
影响因子：
3.9
通讯作者：
Sun, Man Ji
中科院分区：
文献类型：
--
作者：
Li, Xu Ling;Wang, De Sheng;Sun, Man Ji
复智散是一种中草药复方，用于治疗阿尔茨海默病(AD)已有15年以上的历史。以往的研究表明，扶正散可提高AD患者和AD模型大鼠的认知能力。扶正散对Aβ25-35诱导的AD模型小鼠的细胞功能损伤有调节作用，并能减轻β-淀粉样蛋白所致的损伤，并呈剂量依赖性调节和改善胆碱能功能。SPECT显像显示，FZS改善了AD患者额叶、颞叶和穹隆回的血流。然而，有关FZS对自然衰老大鼠影响的研究报道甚少。潜在的机制也有待探索。最近，我们研究了扶正散水提物对老年大鼠认知功能的影响及其治疗作用的药理学基础。结果表明，扶正散(0.3、0.6、1.2g/kg/d)对老年大鼠认知功能损害有明显的改善作用。FZS连续灌胃30d后，大鼠Morris水迷宫潜伏期明显缩短。以氟-18-脱氧葡萄糖(F-18-FDG)(18F-FDG)为示踪剂的微正电子发射断层扫描(MicroPET)显示，扶正舒缓冲剂可促进老年大鼠全脑特别是颞区和顶区的糖代谢。用分光光度法和Western印迹法检测结果表明，扶正散可明显提高老年大鼠海马胆碱O-乙酰基转移酶(ChAT)的活性和产量及乙酰胆碱(ACh)含量，从而调节和改善老年大鼠胆碱能功能受损。扶正散对老龄大鼠学习记忆的影响呈剂量依赖性。扶正散改善老年大鼠记忆功能障碍的作用机制可能与增强脑胆碱能系统功能，促进脑内糖代谢有关。这项研究的结果，以及对FZS临床治疗结果的调查表明，FZS不仅可以缓解痴呆症的症状，还可能增加神经递质ACh的产生和大脑中的能量供应，以建立患者的嘴唇适合性。FZS可能有益于治疗阿尔茨海默病或认知障碍，老年人。(C)2008年，爱思唯尔公司出版。
Fuzhisan (FZS), a Chinese herbal complex prescription, has been used in the treatment of Alzheimer's disease (AD) for more than 15 years. Previous studies showed that FZS enhanced the cognitive ability in AD patients and AD model rats. FZS modulated the impaired cellular functions, and attenuated the damage caused by beta-amyloid protein, dose-dependently regulated and ameliorated the cholinergic functions of the A beta 25-35-induced AD-model mice. The SPECT imaging revealed that FZS improved the blood flow of the frontal and temporal lobes and the callosal gyrus in AD patients. However, little investigation of the effects of FZS on the naturally aged rats was reported. The underlying mechanism also remains to be explored. Recently we investigated the effects of the aqueous extract of FZS on the cognitive functions of the aged rats and the pharmacological basis for its therapeutic efficacy. The results showed a significant improvement made by FZS (0.3, 0.6, and 1.2 g/kg/d) for impaired cognitive functions of the aged rats. The rats manifested a shortened latency in Morris water maze test after intra-gavage administration (ig) of FZS for 30 consecutive days. The micro-positron emission tomography (microPET) using F-18-2-fluoro-2-deoxy-D-glucose (F-18-FDG) (18F-FDG) as the tracer demonstrated that FZS promoted the glucose metabolism in the whole brains especially the temporal and parietal regions in the aged rats. The spectrophotometry and Western blot showed that FZS obviously increased the activity and the production of choline O-acetyltransferase (ChAT, EC 2.3.1.6) and the acetylcholine (ACh) contents in the hippocampus, thus regulated and ameliorated the impaired cholinergic functions of the aged rats. The therapeutical effects of FZS on the learning and memory of the aged rats were dose-dependent. The mechanism of action of FZS in ameliorating the memory dysfunction of the aged rats is ascribed to the reinforcement of the function of the cholinergic system and the enhancement of the glucose metabolism in the brains. The results of this study, together with a survey of the findings in the clinical treatment with FZS suggest that FZS may not merely alleviate the symptoms of the dementia, but may also augment the production of the neurotransmitter ACh and the energy supply in the brain to build LIP fitness of the patients. FZS may be beneficial for the treatment of Alzheimer's disease or cognitive impairment it, old people. (C) 2008 Published by Elsevier Inc.