A Cajal body-specific pseudouridylation guide RNA is composed of two box H/ACA snoRNA-like domains
A Cajal body-specific pseudouridylation guide RNA is composed of two box H/ACA snoRNA-like domains
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DOI：
10.1093/nar/gkf592
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发表时间：
2002-11-01
影响因子：
14.9
通讯作者：
Kiss, T
中科院分区：
文献类型：
--
作者：
Kiss, AM;Jády, BE;Kiss, T
核糖体RNA和小核RNA（snRNA）中尿苷到假尿苷的位点特异性转录后转化由向导RNA指导，向导RNA具有保守的盒H和ACA序列元件，并折叠成共有的“发夹-铰链-发夹-尾”二级结构。在这里，我们描述了一种不寻常的哺乳动物pseudouridylation向导RNA，称为U93，这是由两个串联排列的盒H/ACA RNA结构域。因此，U93 RNA携带两个H和两个ACA盒基序，所有这些都是全长RNA积累所必需的。人U93 RNA在Cajal（卷曲）体中积累，并且预测其在U2剪接体snRNA的假尿苷酸化中起作用。我们的研究结果提供了进一步的支持，修改的RNA聚合酶II转录剪接体snRNAs发生在卡哈尔机构的概念。
Site-specific post-transcriptional conversion of uridines to pseudouridine in ribosomal RNAs and small nuclear RNAs (snRNAs) is directed by guide RNAs which possess the conserved box H and ACA sequence elements and fold into the consensus 'hairpin-hinge-hairpin-tail' secondary structure. Here, we describe an unusual mammalian pseudouridylation guide RNA, called U93, that is composed of two tandemly arranged box H/ACA RNA domains. The U93 RNA therefore carries two H and two ACA box motifs, all of which are essential for accumulation of the full-length RNA. The human U93 RNA accumulates in Cajal (coiled) bodies and it is predicted to function in pseudouridylation of the U2 spliceosomal snRNA. Our results lend further support to the notion that modification of the RNA polymerase II-transcribed spliceosomal snRNAs takes place in Cajal bodies.