The female urinary microbiome: a comparison of women with and without urgency urinary incontinence.
The female urinary microbiome: a comparison of women with and without urgency urinary incontinence.
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DOI：
10.1128/mbio.01283-14
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发表时间：
2014-07-08
期刊：
影响因子：
6.4
通讯作者：
Wolfe AJ
中科院分区：
文献类型：
--
作者：
Pearce MM;Hilt EE;Rosenfeld AB;Zilliox MJ;Thomas-White K;Fok C;Kliethermes S;Schreckenberger PC;Brubaker L;Gai X;Wolfe AJ
在没有临床感染的情况下，在人类尿液中检测到了细菌 DNA 和活细菌，这挑战了尿液通常是无菌的普遍观念。急迫性尿失禁（UUI）是一种人们知之甚少的泌尿系统疾病，其特征是与泌尿道感染重叠的症状，包括尿急和尿失禁频率增加。最近对尿液微生物组的发现值得研究细菌是否会导致 UUI。在这项研究中，我们使用 16S rRNA 基因测序对细菌 DNA 进行分类，并使用扩大定量尿液培养 (EQUC) 技术来分离尿液中的活细菌，这些尿液是使用经尿道导管从患有 UUI 的女性和没有 UUI 的女性中收集的。对于这些队列，我们根据序列和培养证据证明，UUI 和非 UUI 尿液微生物组因组别而异。与非 UUI 微生物组相比，测序实验表明，UUI 微生物组由增加的加德纳菌和减少的乳酸菌组成。 UUI 队列中更常培养出九个属（放线菌属、放线菌属、气球菌属、节杆菌属、棒状杆菌属、加德纳菌属、寡菌属、葡萄球菌属和链球菌属）。尽管从两个队列中分离出了乳杆菌，但在物种水平上存在差异，加氏乳杆菌在 UUI 队列中更常见，而卷曲乳杆菌在对照组中最常见。综合起来，这些数据表明，患有和不患有 UUI 的女性的尿液微生物组存在潜在的重要差异，这对 UUI 的预防、诊断或治疗具有重要意义。新证据表明人类泌尿道含有微生物群落；然而，这些群落在泌尿系统健康中的作用仍有待阐明。急迫性尿失禁（UUI）是一种非常普遍但人们知之甚少的泌尿系统疾病，其特征是尿急、尿频和尿失禁。鉴于 UUI 症状与尿路感染症状显着重叠，UUI 可能有微生物成分。我们使用高通量测序和扩展培养技术，将受 UUI 影响的女性的尿液微生物组与未患 UUI 的对照组的尿液微生物组进行了比较。我们发现存在的细菌的频率和丰度存在统计学上的显着差异。这些差异表明尿液微生物组在女性泌尿系统健康中具有潜在作用。
Bacterial DNA and live bacteria have been detected in human urine in the absence of clinical infection, challenging the prevailing dogma that urine is normally sterile. Urgency urinary incontinence (UUI) is a poorly understood urinary condition characterized by symptoms that overlap urinary infection, including urinary urgency and increased frequency with urinary incontinence. The recent discovery of the urinary microbiome warrants investigation into whether bacteria contribute to UUI. In this study, we used 16S rRNA gene sequencing to classify bacterial DNA and expanded quantitative urine culture (EQUC) techniques to isolate live bacteria in urine collected by using a transurethral catheter from women with UUI and, in comparison, a cohort without UUI. For these cohorts, we demonstrated that the UUI and non-UUI urinary microbiomes differ by group based on both sequence and culture evidences. Compared to the non-UUI microbiome, sequencing experiments revealed that the UUI microbiome was composed of increased Gardnerella and decreased Lactobacillus. Nine genera (Actinobaculum, Actinomyces, Aerococcus, Arthrobacter, Corynebacterium, Gardnerella, Oligella, Staphylococcus, and Streptococcus) were more frequently cultured from the UUI cohort. Although Lactobacillus was isolated from both cohorts, distinctions existed at the species level, with Lactobacillus gasseri detected more frequently in the UUI cohort and Lactobacillus crispatus most frequently detected in controls. Combined, these data suggest that potentially important differences exist in the urinary microbiomes of women with and without UUI, which have strong implications in prevention, diagnosis, or treatment of UUI. New evidence indicates that the human urinary tract contains microbial communities; however, the role of these communities in urinary health remains to be elucidated. Urgency urinary incontinence (UUI) is a highly prevalent yet poorly understood urinary condition characterized by urgency, frequency, and urinary incontinence. Given the significant overlap of UUI symptoms with those of urinary tract infections, it is possible that UUI may have a microbial component. We compared the urinary microbiomes of women affected by UUI to those of a comparison group without UUI, using both high-throughput sequencing and extended culture techniques. We identified statistically significant differences in the frequency and abundance of bacteria present. These differences suggest a potential role for the urinary microbiome in female urinary health.