The Tzs protein from Agrobacterium tumefaciens C58 produces zeatin riboside 5′‐phosphate from 4‐hydroxy‐3‐methyl‐2‐(E)‐butenyl diphosphate and AMP
The Tzs protein from Agrobacterium tumefaciens C58 produces zeatin riboside 5′‐phosphate from 4‐hydroxy‐3‐methyl‐2‐(E)‐butenyl diphosphate and AMP
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DOI：
10.1016/s0014-5793(02)03258-1
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发表时间：
2002-09
期刊：
影响因子：
3.5
通讯作者：
L. Krall;Maja Raschke;M. Zenk;C. Baron
中科院分区：
文献类型：
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作者：
L. Krall;Maja Raschke;M. Zenk;C. Baron
植物病原体根癌农杆菌在由来自受伤植物的次级代谢产物诱导毒力基因时产生细胞分裂素，并且这些激素被认为刺激感染过程。为了研究玉米素的生物合成途径，从玉米中克隆了编码玉米素反式合成蛋白的tzs基因。tumefaciens，并过量生产和纯化该蛋白。分析其与放射性标记底物的反应性，证明4-羟基-3-甲基-2-（E）-丁烯基二磷酸（脱氧木酮糖磷酸途径的产物）与AMP转化为玉米素核苷5′-磷酸。这表明A.根癌农杆菌使用细胞分裂素生物合成的替代途径，该途径先前被假设在植物中起作用。
The plant pathogen Agrobacterium tumefaciens produces cytokinins upon induction of the virulence genes by secondary metabolites from wounded plants, and these hormones are believed to stimulate the infection process. To study the biosynthetic pathway, the tzs gene, encoding the Tzs (trans-zeatin-synthesizing) protein from A. tumefaciens, was cloned and the protein was overproduced and purified. Analysis of its reactivity with radioactively labeled substrate demonstrated conversion of 4-hydroxy-3-methyl-2-(E)-butenyl diphosphate, a product of the deoxyxylulose phosphate pathway, with AMP to zeatin riboside 5′-phosphate. This suggests that A. tumefaciens uses an alternative pathway of cytokinin biosynthesis, which had previously been hypothesized to operate in plants.