Water residing in small ultrastructural spaces plays a critical role in the mechanical behavior of bone.
Water residing in small ultrastructural spaces plays a critical role in the mechanical behavior of bone.
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DOI：
10.1016/j.bone.2013.11.018
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发表时间：
2014-02
期刊：
影响因子：
4.1
通讯作者：
Wang, Xiaodu
中科院分区：
文献类型：
--
作者：
Samuel, Jitin;Sinha, Debarshi;Zhao, John Cong-Gui;Wang, Xiaodu
水可以通过两种主要途径与矿物相和有机相相互作用来影响骨的力学行为：即氢键和极性相互作用。在本研究中，将脱水骨浸泡在几种溶剂（即水、重水（D2O）、乙二醇（EG）、二甲基甲酰胺（DMF）和四氯化碳（CCl4））中，这些溶剂对骨化学无害，极性、氢键能力和分子大小不同。目的是检查用溶剂替代原始基质水将如何影响骨的机械行为。浸泡在这些溶剂中的骨标本的力学性能进行了测量，在张力的渐进加载方案。此外，未进行任何处理的骨样本作为基线对照进行测试，而脱水骨样本作为阴性对照。实验结果表明，CCl_4、DMF、EG、D_2O和H_2O可分别置换骨中22.3±5.17vol%、71.8±3.77vol%、85.5±5.15vol%和近100%的基质水。CCl4浸泡试样的力学性能与脱水试样相似。尽管在替换水方面存在很大差异，但与脱水骨样本相比，EG和DMF浸泡样本的力学行为仅存在轻微差异。相比之下，D2O保留了与水相当的骨的机械性能。本研究的结果表明，有限部分的水（<原始基质水的15vol%）在骨的机械行为中起着关键作用，并且它最有可能存在于小的基质空间中，大于4.0 μ l的溶剂分子无法渗透到其中。
Water may affect the mechanical behavior of bone by interacting with the mineral and organic phases through two major pathways: i.e. hydrogen bonding and polar interactions. In this study, dehydrated bone was soaked in several solvents (i.e. water, heavy water (D2O), ethylene glycol (EG), dimethylformamide (DMF), and carbon tetrachloride(CCl4)) that are chemically harmless to bone and different in polarity, hydrogen bonding capability and molecular size. The objective was to examine how replacing the original matrix water with the solvents would affect the mechanical behavior of bone. The mechanical properties of bone specimens soaked in these solvents were measured in tension in a progressive loading scheme. In addition, bone specimens without any treatments were tested as the baseline control whereas the dehydrated bone specimens served as the negative control. The experimental results indicated that 22.3±5.17vol% of original matrix water in bone could be replaced by CCl4, 71.8±3.77vol% by DMF, 85.5±5.15vol% by EG, and nearly 100% by D2O and H2O, respectively. CCl4 soaked specimens showed similar mechanical properties with the dehydrated ones. Despite of great differences in replacing water, only slight differences were observed in the mechanical behavior of EG and DMF soaked specimens compared with dehydrated bone samples. In contrast, D2O preserved the mechanical properties of bone comparable to water. The results of this study suggest that a limited portion of water (<15vol% of the original matrix water) plays a pivotal role in the mechanical behavior of bone and it most likely resides in small matrix spaces, into which the solvent molecules larger than 4.0Å cannot infiltrate.
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