Simultaneous Cr(VI) reduction and Zn(II) biosorption by Stenotrophomonas sp and constitutive expression of related genes
Simultaneous Cr(VI) reduction and Zn(II) biosorption by Stenotrophomonas sp and constitutive expression of related genes
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DOI：
10.1007/s10529-016-2057-8
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发表时间：
2016-05-01
影响因子：
2.7
通讯作者：
Ge, Shi Chao
中科院分区：
文献类型：
--
作者：
Ge, Shimei;Ge, Shi Chao
研究微生物同时进行的 Cr(VI) 还原和 Zn(II) 生物吸附。一种新的分离株，寡养单胞菌 sp。 TD3，同时还原Cr(VI)并实现Zn(II)生物吸附。该菌株对其他金属和盐类具有抗性，并且CaCl2和MgCl2可以极大地刺激其Zn生物吸附，这在以前没有报道过。使用定量实时 PCR 扩增和测量三个假定的 Cr 或 Zn 相关基因，chrR、zupT 和 hmrE，以评估它们在寡养单胞菌中的 mRNA 表达水平。这三个假定基因对 Cr6+、Zn2+、Ca2+ 和 Mg2+ 不敏感，这表明它们在寡养单胞菌中组成型表达。 TD3。这些结果提高了我们对该机制的理解，并表明该菌株是促进 Cr(VI) 和 Zn 污染场地修复的潜在候选菌株。
To investigate simultaneous Cr(VI) reduction and Zn(II) biosorption by a microorganism.A new isolate, Stenotrophomonas sp. TD3, simultaneously reduced Cr(VI) and achieved Zn(II) biosorption. The strain was resistant to other metals and salts, and its Zn biosorption could be stimulated greatly by CaCl2 and MgCl2, which has not been reported previously. Three putative Cr- or Zn-related genes, chrR, zupT and hmrE, were amplified and measured using quantitative real-time PCR to evaluate their mRNA expression levels in Stenotrophomonas sp. The three putative genes were not sensitive to Cr6+, Zn2+, Ca2+, and Mg2+, which suggested that they were constitutively expressed in Stenotrophomonas sp. TD3.These results improve our understanding of the mechanism and suggest that the strain is a potential candidate for facilitating remediation of Cr(VI) and Zn contaminated sites.