Higher concentration of transforming growth factor-β in aqueous humor of glaucomatous eyes and diabetic eyes
Higher concentration of transforming growth factor-β in aqueous humor of glaucomatous eyes and diabetic eyes
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DOI：
10.1016/s0021-5155(01)00523-8
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发表时间：
2002-05-01
影响因子：
2.4
通讯作者：
Ochiai, H
中科院分区：
文献类型：
--
作者：
Ochiai, Y;Ochiai, H
目的：糖尿病视网膜病变和青光眼是后天性失明的主要原因。包括转化生长因子（TGF）-β在内的细胞因子可能参与这些疾病。因此，我们收集了青光眼和/或糖尿病患者接受手术，并测定了TGF-β的浓度。方法：收集了80例患者（84只眼），其中原发性开角型青光眼（POAG）19只眼，糖尿病22只眼，糖尿病合并POAG 18只眼的房水样本。25只白内障眼作为对照。结果：各组TGF-β 1浓度均小于0.1pg/mL;与对照组的1001.4 +/- 444.1 pg/mL相比，糖尿病组的总TGF-β 2浓度为1715.6 +/- 882.1 pg/mL，糖尿病合并POAG组为1692.9 +/- 361.9 pg/mL。这些都显着高于对照组。与对照组（321.2 +/- 197.9 pg/mL）相比，POAG患者的成熟TGF-β 2浓度为822.5 +/- 484.4 pg/mL，糖尿病合并POAG患者的成熟TGF-β 2浓度为1058.9 +/- 648.4 pg/mL。这些都显着高于对照组。结论：高浓度的总TGF-β 2和成熟TGF-β 2可能与POAG、糖尿病及糖尿病合并POAG的进展有关。(C)2002年日本眼科学会。
Purpose: Diabetic retinopathy and glaucoma are the primary Causes of acquired blindness. Cytokines including transforming growth factor (TGF)-beta may be involved in these diseases. We therefore collected aqueous humor samples from patients with glaucoma and/or diabetes who were undergoing surgery, and determined the concentration of TGF-beta.Methods: Aqueous humor samples were collected from 80 patients (84 eyes), including 19 eyes with primary open-angle glaucoma (POAG), 22 eyes with diabetes, and 18 eyes with diabetes complicated with POAG. Twenty-five eyes with cataract served as controls. The concentration of TGF-beta1 or TGF-beta2 was measured by enzyme-linked immunosorbent assay.Results: The concentration of TGF-beta1 was less than 0.1 pg/mL in all of the groups. In contrast to controls who had 1001.4 +/- 444.1 pg/mL, the concentration of total TGF-beta2 in the diabetes group was 1715.6 +/- 882.1 pg/mL, and that in the diabetes complicated with POAG group was 1692.9 +/- 361.9 pg/mL. These were significantly higher than that in controls. In contrast to the controls who had 321.2 +/- 197.9 pg/mL, the concentration of mature TGF-beta2 with POAG was 822.5 +/- 484.4 pg/mL, and that of diabetes complicated with POAG was 1058.9 +/- 648.4 pg/mL. These were significantly higher than that in the controls. The eyes with diabetes complicated with POAG also had a significantly higher concentration than the eyes with diabetes alone.Conclusion: Total TGF-beta2 and mature TGF-beta2 in high concentration may correlate with progression of POAG, diabetes, and diabetes complicated with POAG. (C) 2002 Japanese Ophthalmological Society.