Spider Mites Singly Infected With Either Wolbachia or Spiroplasma Have Reduced Thermal Tolerance.
Spider Mites Singly Infected With Either Wolbachia or Spiroplasma Have Reduced Thermal Tolerance.
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单独感染沃尔巴克氏体或螺原体的蜘蛛螨的耐热性降低
DOI：
10.3389/fmicb.2021.706321
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发表时间：
2021
影响因子：
5.2
通讯作者：
Hong XY
中科院分区：
文献类型：
--
作者：
Zhu YX;Song ZR;Zhang YY;Hoffmann AA;Hong XY
可遗传共生体在寄主生态学的许多方面以温度依赖的方式发挥着重要作用。然而，温度如何影响宿主及其与内共生体的相互作用在很大程度上仍然未知。在这里，我们研究了中等温度（20°C）和高温（30和35°C）对截形叶螨和两种母系遗传内共生体（Wolbachia和Spiroplasma）之间共生的影响。我们发现，螨的热耐受性（通过热暴露后的存活率来衡量）对于单独感染沃尔巴克氏体或螺原体的螨比共感染或未感染的螨要低。虽然认为相对较高的温度（30°C）促进细菌复制，但在高温（35°C）下饲养导致沃尔巴克氏体和特别是螺原体的损失。将螨暴露于20°C降低了螺原体的密度和传播，但Wolbachia没有。四种红蜘蛛品系在中高温下诱导的热休克基因（Hsps）数目不同。在热偏好（Tp）测定中，两个Wolbachia感染的红蜘蛛菌株喜欢较低的温度比菌株没有Wolbachia。我们的研究结果表明，内共生体介导的红蜘蛛对温度胁迫的反应是复杂的，涉及到改变内共生体感染模式，改变体温调节行为和转录反应的组合。
Heritable symbionts play an essential role in many aspects of host ecology in a temperature-dependent manner. However, how temperature impacts the host and their interaction with endosymbionts remains largely unknown. Here, we investigated the impact of moderate (20°C) and high (30 and 35°C) temperatures on symbioses between the spider mite Tetranychus truncatus and two maternally inherited endosymbionts (Wolbachia and Spiroplasma). We found that the thermal tolerance of mites (as measured by survival after heat exposure) was lower for mites that were singly infected with either Wolbachia or Spiroplasma than it was for co-infected or uninfected mites. Although a relatively high temperature (30°C) is thought to promote bacterial replication, rearing at high temperature (35°C) resulted in losses of Wolbachia and particularly Spiroplasma. Exposing the mites to 20°C reduced the density and transmission of Spiroplasma but not Wolbachia. The four spider mite strains tested differed in the numbers of heat shock genes (Hsps) induced under moderate or high temperature exposure. In thermal preference (Tp) assays, the two Wolbachia-infected spider mite strains preferred a lower temperature than strains without Wolbachia. Our results show that endosymbiont-mediated spider mite responses to temperature stress are complex, involving a combination of changing endosymbiont infection patterns, altered thermoregulatory behavior, and transcription responses.
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影响因子：
2.2
作者：
Montllor, CB;Maxmen, A;Purcell, AH
通讯作者：
Purcell, AH
影响因子：
5.2
作者：
Dahlgaard, J;Loeschcke, V;Justesen, J
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Justesen, J
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6.7
作者：
Doremus, Matthew R.;Kelly, Suzanne E.;Hunter, Martha S.
通讯作者：
Hunter, Martha S.
影响因子：
3
作者：
Heyworth, Eleanor R.;Smee, Melanie R.;Ferrari, Julia
通讯作者：
Ferrari, Julia
影响因子：
4
作者：
Brumin, Marina;Kontsedalov, Svetlana;Ghanim, Murad
通讯作者：
Ghanim, Murad