SCHISTOSOMA-MANSONI - IDENTIFICATION AND ANALYSIS OF AN MESSENGER-RNA AND A GENE ENCODING SUPEROXIDE-DISMUTASE (CU/ZN)
SCHISTOSOMA-MANSONI - IDENTIFICATION AND ANALYSIS OF AN MESSENGER-RNA AND A GENE ENCODING SUPEROXIDE-DISMUTASE (CU/ZN)
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DOI：
10.1016/0014-4894(88)90010-0
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发表时间：
1988-10-01
影响因子：
2.1
通讯作者：
LOVERDE, PT
中科院分区：
文献类型：
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作者：
SIMURDA, MC;VANKEULEN, H;LOVERDE, PT
曼氏血吸虫超氧化物歧化酶（EC 1.15.1.1）的mRNA序列已被测定。它编码的推导的氨基酸序列与来自10个其它物种的胞质超氧化物歧化酶的氨基酸序列显示出40%至45%的同源性，以及与人细胞外形式的相似同源性。通过cDNA克隆pSM 38对mRNA的杂交选择和该mRNA的体外翻译一直产生20，000 Da的蛋白质，偶尔产生40，000 Da的蛋白质。这两种抗体都能与慢性血吸虫病患者的血清发生免疫沉淀。转录这种mRNA的基因也已被分离和表征。它跨越5.1 kb的染色体DNA，具有三个外显子和两个内含子，中断编码区。这些内含子含有剪接点序列，其适合在哺乳动物基因中观察到的共有序列。该基因的上游序列显示了一个潜在的“TATA”盒区域，下游序列包含两个多聚腺苷酸化位点。
The sequence of an mRNA encoding superoxide dismutase (EC 1.15.1.1) of Schistosoma mansoni has been determined. The deduced amino acid sequence which it encodes shows 40 to 45% homology with the amino acid sequences of the cytosolic superoxide dismutase from 10 other species, as well as similar homology with the human extracellular form. Hybridization selection of the mRNA by cDNA clone pSM38 and in vitro translation of this mRNA have consistently produced a protein of 20,000 Da and occasionally, a 40,000-Da protein. Both are immunoprecipitable with sera pooled from patients with chronic schistosomiasis. The gene from which this mRNA is transcribed has also been isolated and characterized. It spans 5.1 kb of chromosomal DNA and possesses three exons and two introns which interrupt the coding region. These introns contain the splice junction sequences which fit the consensus sequences observed in mammalian genes. The upstream sequence of the gene shows a region for a potential ''TATA'' box and the downstream sequence contains two polyadenylation sites.