Characterisation of the bacterial community in expressed prostatic secretions from patients with chronic prostatitis/chronic pelvic pain syndrome and infertile men: a preliminary investigation
Characterisation of the bacterial community in expressed prostatic secretions from patients with chronic prostatitis/chronic pelvic pain syndrome and infertile men: a preliminary investigation
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DOI：
10.1038/aja.2012.30
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发表时间：
2012-07-01
影响因子：
2.9
通讯作者：
Xu, Chen
中科院分区：
文献类型：
--
作者：
Hou, Dong-Sheng;Long, Wen-Min;Xu, Chen
对慢性前列腺炎/慢性盆腔疼痛综合征（CP/CPPS）男性、不育男性和正常男性的前列腺分泌物（EPS）进行微生物学研究。收集受试者的 EPS，其中包括 26 名正常男性、11 名不育患者和 51 名 CP/CPPS 患者。从每个样本中提取 DNA，并使用通用细菌引物扩增 16S rRNA 基因的 V3 区域。结果显示，CP/CPPS和不育患者的EPS 16S rRNA基因阳性率远高于正常男性，但三组患者之间无差异。使用变性梯度凝胶电泳 (DGGE) 方法来表征 CP/CPPS 或不孕问题患者的前列腺液中 EPS 细菌群落结构。 PCR-DGGE 图谱的主成分分析 (PCA) 和偏最小二乘 (PLS) 分析表明，三组之间的 EPS 细菌群落结构不同。三个条带被确定为造成 CP/CPPS 患者与不孕患者之间差异的关键因素 (P
The expressed prostatic secretions (EPSs) of men with chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS), infertile men and normal men were subjected to microbiological study. EPSs were collected from the subjects, which included 26 normal men, 11 infertile patients and 51 CP/CPPS patients. DNA was extracted from each specimen, and the V3 regions of the 16S rRNA genes were amplified using universal bacterial primers. The results showed that the EPS 16S rRNA gene-positive rate in the CP/CPPS and infertile patients was much higher than in the normal men, but without any difference among the three patient groups. The denaturing gradient gel electrophoresis (DGGE) method was used to characterize the EPS bacterial community structure of the prostate fluid from patients with CP/CPPS or infertility issues. Principal component analysis (PCA) and partial least squares (PLS) analyses of PCR-DGGE profiles revealed that the EPS bacterial community structure differed among the three groups. Three bands were identified as the key factors responsible for the discrepancy between CP/CPPS patients and infertile patients (P