Identification of the protein encoded by the human diffuse B-cell lymphoma (dbl) oncogene.
Identification of the protein encoded by the human diffuse B-cell lymphoma (dbl) oncogene.
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鉴定人弥漫性 B 细胞淋巴瘤 (dbl) 癌基因编码的蛋白质。
DOI：
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发表时间：
1986
影响因子：
11.1
通讯作者：
A. Eva
中科院分区：
文献类型：
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作者：
S. Srivastava;R. Wheelock;S. Aaronson;A. Eva
DBL癌基因最初是从人类弥漫性B细胞淋巴瘤中分离出来的。由该癌基因诱导的荷瘤小鼠的抗血清在DBL转化子中特异性地检测到约66 kDa(P66)的蛋白质。从表达p66的转基因克隆中分离到的DBLcDNA选择的Poly(A)+RNA指导了该蛋白的体外合成，证实它是由DBL编码的。亚细胞定位研究表明，p66是一种胞质蛋白，分布于胞浆和粗膜部分之间。此外，p66被证明是一种磷酸化蛋白，丝氨酸残基具有特异性的磷酸化。我们对DBL编码蛋白的表征似乎将这个转化基因产物与其他已知癌基因的产物区分开来。
The dbl oncogene was initially isolated from a human diffuse B-cell lymphoma. Antisera from mice bearing tumors induced by this oncogene specifically detected a protein of about 66 kDa (p66) in dbl transformants. dbl cDNA-selected poly(A)+ RNA isolated from a transfectant clone expressing p66 directed the in vitro synthesis of this protein, establishing that it is encoded by dbl. Subcellular localization studies revealed that p66 is a cytoplasmic protein distributed between cytosol and crude membrane fractions. Moreover, p66 was shown to be a phosphoprotein, with phosphorylation specific to serine residues. Our characterization of the dbl-encoded protein appears to distinguish this transforming gene product from those of other known oncogenes.