Spray Performance of Microfluidic Glass Devices with Integrated Pulled Nanoelectrospray Emitters
Spray Performance of Microfluidic Glass Devices with Integrated Pulled Nanoelectrospray Emitters
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DOI：
10.1021/ac9015038
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发表时间：
2009-09-01
影响因子：
7.4
通讯作者：
Belder, Detlev
中科院分区：
文献类型：
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作者：
Hoffmann, Peter;Eschner, Markus;Belder, Detlev
具有单片集成纳米喷雾头的微流体玻璃装置的性能已得到评估。通过拉动步骤，然后进行高频蚀刻，直接在微流控玻璃芯片的边缘上生成纳米喷雾尖端，以定向形成具有限定发射器区域的尖锐尖端。为了公平判断 MS 检测灵敏度，我们将检测性能与商用纳喷雾针进行了比较。为此，详细研究了不同微流控芯片以及商业纳喷雾针的发射器开口对灵敏度的影响。芯片纳米喷雾装置与商业纳米喷雾针的比较表明，在相似的发射器直径下获得了相当的喷雾性能。可以在这种锥形尖端处产生稳定的电喷雾，而不需要任何水动力或电渗泵送。纳米喷雾芯片成功应用于耦合微芯片电泳和质谱分析。为了提高性能，微装置的分离通道用羟丙基甲基纤维素 (HPMC) 冲洗，以减少分析物与壁的相互作用和电致动流动。
The performance of microfluidic glass devices with a monolithically integrated nanospray tip have been evaluated. The nanospray tip is generated directly on the edge of a microfluidic glass chip by a pulling step followed by HF-etching for directed formation of a sharp tip with defined emitter area. For a fair judgment of the MS-detection sensitivity, we compared the detection performance with commercial nanospray needles. For that purpose the effect of the emitter opening on the sensitivity was studied in detail for different microfluidic chips as well as for commercial nanospray needles. A comparison of the chip-nanospray device with commercial nanospray needles revealed that a comparable spray performance is obtained at similar emitter diameters. A stable electrospray could be generated at such tapered tips without any need for hydrodynamic or electroosmotic pumping. The nanospray chips were successfully applied for coupling microchip electrophoresis and mass spectrometry. For improved performance, the separation channel of the microdevice was flushed with hydroxypropylmethylcellulose (HPMC) in order to reduce analyte-wall interactions and electroostriotic flow.