Planar relationships of the semicircular canals in two strains of mice
Planar relationships of the semicircular canals in two strains of mice
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DOI：
10.1007/s10162-006-0031-1
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发表时间：
2006-06-01
影响因子：
2.4
通讯作者：
Hullar, Timothy E.
中科院分区：
文献类型：
--
作者：
Calabrese, Daniel R.;Hullar, Timothy E.
小鼠作为前庭研究的对象越来越重要。虽然许多研究都集中在前庭反应的小鼠角旋转，其半规管的几何形状还没有被描述。采用高压X线计算机断层扫描技术对C57 BI/6 J和CBA/CaJ两种小鼠半规管进行解剖学测量。立体定位坐标系的水平面由外耳道的中点和切牙从上颌骨出现的点定义。计算骨管内腔的质心，并使用最小二乘回归分析将描述骨管的平面拟合到所得点。垂直于每个回归平面的矢量被用来代表相应的耳道的旋转轴，并计算这些矢量相对于颅骨标志以及彼此之间的角度。发现小鼠的水平管从参考水平面向前向上倾斜17.8度（CBA/CaJ）和32.6度（C57 B1/6 J）。同侧管之间的角度偏离正交高达12.3度，对侧协同管之间的角度（左前-右后，右前-左后，和水平-水平）偏离平行高达14.8度。两种品系之间的头部内的运河的方向以及运河相对于彼此的方向是显着不同的，这表明，必须小心的设计和解释的发展和生理研究，涉及不同的小鼠品系。
The mouse is increasingly important as a subject of vestibular research. Although many studies have focused on the vestibular responses of mice to angular rotation, the geometry of their semicircular canals has not been described. High-voltage X-ray computed tomography was used to measure the anatomy of the semicircular canals of two strains of mice, C57BI/6J and CBA/CaJ. The horizontal plane of a stereotaxic coordinate system was defined by the midpoints of the external auditory meati and the point where the incisors emerge from the maxilla. The centroids of the lumens of the bony canals were calculated, and planes that describe the canals were fit using a least-squares regression analysis to the resulting points. Vectors normal to each regressed plane were used to represent the corresponding canal's axis of rotation, and angles of these vectors relative to skull landmarks as well as to each other were calculated. The horizontal canal of the mouse was found to be angled anteriorly upward 17.8 degrees for CBA/CaJ and 32.6 degrees for C57B1/6J from the reference horizontal plane. Angles between ipsilateral canals deviated up to 12.3 degrees from orthogonal, and angles between contralateral synergistic canals (left anterior-right posterior, right anterior-left posterior, and horizontal-horizontal) deviated from parallel by up to 14.8 degrees. The orientations of the canals within the head as well as the orientations of the canals relative to each other were significantly different between the two strains, suggesting that care must be taken in the design and interpretation of developmental and physiologic studies involving different mouse strains.