Proteomic analysis of mitochondria from Caenorhabditis elegans
Proteomic analysis of mitochondria from Caenorhabditis elegans
复制标题
秀丽隐杆线虫线粒体的蛋白质组学分析
DOI：
10.1002/pmic.200900101
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发表时间：
2009-10-01
期刊：
影响因子：
3.4
通讯作者：
Yang, Fuquan
中科院分区：
文献类型：
--
作者：
Li, Jing;Cai, Tanxi;Yang, Fuquan
线粒体在细胞能量产生、代谢、离子稳态、细胞生长、衰老和凋亡等生理过程中起着重要作用。自1998年以来，蛋白质组学策略已被应用于线粒体的研究;这些研究已经产生了关于细胞器的多种生理功能的决定性信息。秀丽隐杆线虫作为一种理想的模式生物系统，已被广泛应用于代谢性疾病、癌症等多种疾病的研究。然而，C.秀丽线虫仍然难以捉摸。在这项研究中，我们从C.并使用鸟枪蛋白质组学方法进行了全面的蛋白质组学分析。总共有1117种蛋白质被鉴定出至少有两种独特的肽。他们的物理化学和功能特性，亚细胞位置，相关的生物过程，并与人类疾病，特别是帕金森氏病的协会进行了讨论。并对C. elegans和其他四种模式生物的线粒体蛋白质的保护在进化过程中的一般描述。本研究将为了解线粒体在C.优雅的。
Mitochondria play essential roles in cell physiological processes including energy production, metabolism, ion homeostasis, cell growth, aging and apoptosis. Proteomic strategies have been applied to the study of mitochondria since 1998; these studies have yielded decisive information about the diverse physiological functions of the organelle. As an ideal model biological system, the nematode Caenorhabditis elegans has been widely used in the study of several diseases, such as metabolic diseases and cancer. However, the mitochondrial proteome of C. elegans remains elusive. In this study, we purified mitochondria from C. elegans and performed a comprehensive proteomic analysis using the shotgun proteomic approach. A total of 1117 proteins have been identified with at least two unique peptides. Their physicochemical and functional characteristics, subcellular locations, related biological processes, and associations with human diseases, especially Parkinson's disease, are discussed. An orthology comparison was also performed between C. elegans and four other model organisms for a general depiction of the conservation of mitochondrial proteins during evolution. This study will provide new clues for understanding the role of mitochondria in the physiological and pathological processes of C. elegans.