Degeneration and regeneration in rabbit peripheral nerve with long-term nerve cuff electrode implant: a stereological study of myelinated and unmyelinated axons
Degeneration and regeneration in rabbit peripheral nerve with long-term nerve cuff electrode implant: a stereological study of myelinated and unmyelinated axons
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DOI：
10.1007/s004010050907
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发表时间：
1998-10-01
影响因子：
12.7
通讯作者：
Sinkjaer, T
中科院分区：
文献类型：
--
作者：
Larsen, JO;Thomsen, M;Sinkjaer, T
选择性和动态协调的功能性电刺激（FES）的瘫痪/麻痹肢体上运动神经元损伤的人取决于在神经接口的最佳接触。植入的神经袖带电极可以形成稳定的电神经界面，但也可能造成神经损伤。在这项研究中，直接和长期的影响袖植入的数量和大小的有髓和无髓轴突进行了评估与公正的体视学技术。将袖状电极植入兔膝关节正下方胫神经，并在植入后2周和16个月进行体视学分析。在袖带近端、下方和远端的标准化水平分析有髓轴突;袖带下方的无髓轴突。术后2周，在神经袖带下方和远端发现27%的有髓轴突丢失。除了最小的轴突外，所有轴突的大小都同样丢失。术后16个月，两个节段的有髓轴突数量均恢复至对照值。除术后2周出现再生芽外，术后2周或16个月无髓鞘轴突的数量或大小均无变化。我们的研究表明，植入袖状电极可能会导致有髓轴突的最初损失，但随后再生。
Selective and dynamically co-ordinated functional electrical stimulation (FES) of paralysed/paretic limbs in upper motor neuron lesioned people depends on optimal contact at the neural interface. Implanted nerve cuff electrodes may form a stable electrical neural interface, but may also inflict nerve damage. In this study the immediate and long-term effects of cuff implantation on the number and sizes of myelinated and unmyelinated axons have been evaluated with unbiased stereological techniques. Cuff electrodes were implanted in rabbit tibial nerves just below the knee joint, and the stereological analyses were carried out 2 weeks and 16 months after implantation. Myelinated axons were analysed at standardised levels proximal to, underneath, and distal to the cuff; unmyelinated axons underneath the cuff. A 27% loss of myelinated axons was found underneath and distal to the nerve cuff 2 weeks post surgery. All axonal sizes were equally lost except for the very smallest. At 16 months post surgery the number of myelinated axons was restored to control values at both levels. Except for the presence of regenerative sprouts at 2 weeks post surgery, no changes in the number or sizes of unmyelinated axons were revealed at either 2 weeks or 16 months post surgery. Our study demonstrates that implanted cuff electrodes may cause an initial loss of myelinated axons but with subsequent regeneration.