Siamois is required for formation of Spemann's organizer
Siamois is required for formation of Spemann's organizer
复制标题
DOI：
10.1073/pnas.94.24.13017
复制
发表时间：
1997-11-25
影响因子：
11.1
通讯作者：
Kessler, DS
中科院分区：
文献类型：
--
作者：
Kessler, DS
Spemann的组织者的发展是对通过wnt通路的母体成分起作用的背侧决定因素的反应。siamois，wnt诱导的同源异型盒基因，在Spemann的组织者发展的功能进行了检查，通过融合定义的转录调控结构域的siamois同源结构域。与天然siamois相似，VP 16激活因子融合诱导轴形成，表明siamois在轴诱导中起转录激活因子的作用。融合的enrailed阻遏物产生了一个显性抑制剂，阻断轴诱导Xwnt 8，β-连环蛋白，和siamois，并抑制wnt激活的goosecoid启动子。背部注射的enrailed-siamois融合导致背部发育和组织者基因表达的完全抑制，siamois拯救的效果，但不是由Xwnt 8或β-连环蛋白。因此，作为母亲的背信号合子调解人，siamois功能是必需的发展Spemann的组织者。
Spemann's organizer develops in response to dorsal determinants that act via maternal components of the wnt pathway. The function of siamois, a wnt-inducible homeobox gene, in Spemann's organizer development was examined by fusion of defined transcriptional regulatory domains to the siamois homeodomain. Similar to native siamois, a VP16 activator fusion induced axis formation, indicating that siamois functions as a transcriptional activator in axis induction. Fusion of the engrailed repressor generated a dominant inhibitor that blocked axis induction by Xwnt8, beta-catenin, and siamois, and repressed wnt activation of the goosecoid promoter. Dorsal injection of the engrailed-siamois fusion resulted in complete inhibition of dorsal development and organizer gene expression, an effect rescued by siamois, but not by Xwnt8 or beta-catenin. Thus, as a zygotic mediator of maternal dorsal signals, siamois function is required for development of Spemann's organizer.