Recoding the metagenome: microbiome engineering in situ.
Recoding the metagenome: microbiome engineering in situ.
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DOI：
10.1016/j.mib.2019.09.005
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发表时间：
2019-08
影响因子：
5.4
通讯作者：
Travis Whitfill;Julia Oh
中科院分区：
文献类型：
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作者：
Travis Whitfill;Julia Oh
重点原位微生物组工程允许在不需要稳定整合新生物体的情况下修改微生物组。合成修饰的微生物和接合供体可以将遗传有效载荷转移到微生物组中的不同物种。这些有效载荷可以消除微生物，使病原体对抗生素重新敏感，降低毒力或产生治疗剂。需要基因组，成像和生态学研究来了解功效，影响，合成生物学为微生物组工程设计提供了新一代工具，包括靶向抗生素、蛋白质递送、活体生物传感器和诊断以及代谢工厂。在这里，我们讨论了微生物组工程的机会和局限性，重点是新一代的工具，原位遗传修饰的微生物组，特别是在规避这些限制的承诺。
HighlightsIn situ microbiome engineering allows modification of a microbiome without requiring stable integration of a new organism.Synthetically modified phages and conjugative donors can divert genetic payloads to different species in a microbiome.Such payloads can eliminate microbes, re-sensitize pathogens to antibiotics, reduce virulence, or produce therapeutics.Genomic, imaging, and ecological studies are needed to understand efficacy, impact, and containment of in situ modifications.Synthetic biology has enabled a new generation of tools for engineering the microbiome, including targeted antibiotics, protein delivery, living biosensors and diagnostics, and metabolic factories. Here, we discuss opportunities and limitations in microbiome engineering, focusing on a new generation of tools for in situ genetic modification of a microbiome that hold particular promise in circumventing these limitations.