HPLC-DAD-ESI-MSn identification of phenolic compounds in cultivated strawberries from Macedonia
HPLC-DAD-ESI-MSn identification of phenolic compounds in cultivated strawberries from Macedonia
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DOI：
10.20450/mjcce.2010.165
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发表时间：
2010-12
影响因子：
1
通讯作者：
Marina Kajdžanoska;V. Gjamovski;M. Stefova
中科院分区：
文献类型：
--
作者：
Marina Kajdžanoska;V. Gjamovski;M. Stefova
草莓果实含有酚类化合物，具有抗氧化、抗癌、抗动脉粥样硬化、抗炎和抗神经变性的特性。采用高效液相色谱（HPLC）-电喷雾质谱（ESI-MS）联用技术，以正离子和负离子模式对马其顿共和国16个草莓品种果实中的酚类化合物进行了鉴定。光电二极管阵列检测（DAD）已被用于筛选不同类别的酚类化合物，而MS和MSn碎片数据被用于其结构表征。确定的酚类化合物被分组为：鞣花酸和鞣花酸共轭物与糖，鞣花单宁，花青素，黄酮醇，黄烷醇，和酰化糖（阿魏酰，咖啡酰和香豆酰己糖）。槲皮素和山奈酚是主要的黄酮醇类化合物，分别以槲皮素3-O-葡萄糖苷、槲皮素3-O-葡萄糖苷酸、山奈酚3-O-葡萄糖苷酸、山奈酚3-O-丙二酰葡萄糖苷、山奈酚3-O-乙酰葡萄糖苷和山奈酚3-O-香豆酰葡萄糖苷的形式存在。天竺葵素-3-O-葡萄糖苷是草莓提取物中含量最高的花色苷。原花青素也被确定为二聚体，三聚体和四聚体的（表）儿茶素和（表）afzelechin的MSn片段。这是第一次测定的草莓品种在马其顿，这可以进一步发展的表征和评价其质量方面的酚类成分的酚类配置文件。
Strawberry fruits contain phenolic compounds that exhibit antioxidant, anticancer, antiatherosclerotic, antinflammatory and anti-neurodegenerative properties. High-performance liquid chromatography (HPLC) coupled to electrospray ionization mass spectrometric (ESI-MS) detection in the positive and negative ion mode has been used to identify the phenolic compounds in extracts from sixteen different strawberry cultivar fruits from Republic of Macedonia. Photodiode-array detection (DAD) has been used for screening of the different classes of phenolic compounds, whereas MS and MSn fragmentation data were employed for their structural characterization. The phenolic compounds identified were grouped as: ellagic acid and ellagic acid conjugates with sugars, ellagitannins, anthocyanins, flavonols, flavanols, and acylated sugars (feruloyl, caffeoyl and coumaroyl hexoses). Quercetin and kaempferol were the major flavonols found as quercetin 3-O-glucoside, quercetin 3-O-glucuronide, kaempferol 3-O-glucoside, kaempferol 3-O-glucoronide, kaempferol 3-O-malonylglucoside, kaempferol 3-O-acetylglucoside and kaempferol 3-O-coumaroylglucoside. Pelargonidin-3-O-glucoside was the most abundant anthocyanin in all strawberry extracts. Proanthocyanidins were also identified by MSn fragmentation as dimers, trimers and tetramers of (epi)catechin and (epi)afzelechin. This is the first assay of the phenolic profile of the strawberry cultivars in Macedonia, which can be further developed for characterization and evaluation of their quality with regards to their phenolic composition.