11β-hydroxysteroid dehydrogenase types 1 and 2 in postnatal development of rat testis: gene expression, localization and regulation by luteinizing hormone and androgens.
11β-hydroxysteroid dehydrogenase types 1 and 2 in postnatal development of rat testis: gene expression, localization and regulation by luteinizing hormone and androgens.
复制标题
11 β-羟基类固醇脱氢酶 1 型和 2 型在大鼠睾丸出生后发育中的作用：黄体生成素和雄激素的基因表达、定位和调节
DOI：
10.4103/1008-682x.132471
复制
发表时间：
2014-11
影响因子：
2.9
通讯作者：
Ge RS
中科院分区：
文献类型：
--
作者：
Zhou HY;Chen XX;Lin H;Fei AL;Ge RS
11β-羟基类固醇脱氢酶1(11β-HSD1)和11β-HSD2在大鼠睾丸中表达，调节局部糖皮质激素的浓度。在此，我们研究了11个β-HSD基因在出生后发育过程中大鼠睾丸中的表达和定位，以及黄体生成素和雄激素对这些基因的调控。分别于出生后7、14、21、35、90天采集大鼠睾丸组织，用实时定量聚合酶链式反应和免疫印迹法检测睾丸组织中的基因和蛋白表达水平，并在出生后21天和90天分别给予黄体生成素、7-甲基-19-去甲睾酮和睾酮治疗。免疫组织化学染色鉴定11个β-HSD在PND7、14和90天大鼠睾丸中的定位。我们发现11个β-HSD1的表达仅局限于间质区域，并且在大鼠睾丸发育过程中其表达水平升高。相反，11β-HSD2在PND7的间质和生精小管中均有表达，而在PND90只在间质区域表达，并在睾丸发育过程中水平下降。此外，黄体生成素在PND21、90和PND21的睾丸中均有11个β-HSD1mRNA的表达，而在PND21和PND90的睾丸中，有11个β-HSD2mRNA被睾酮和黄体生成素诱导。总之，我们的研究表明，11个β-hsd1和11个β-hsd2基因在大鼠睾丸出生后发育过程中具有不同的时空表达和激素调节模式。
11β-hydroxysteroid dehydrogenase type 1 (11β-HSD1) and type 2 (11β-HSD2) are expressed in rat testis, where they regulate the local concentrations of glucocorticoids. Here, we investigated the expression and localization of 11β-HSD in rat testis during postnatal development, and the regulation of these genes by luteinizing hormone (LH) and androgens. mRNA and protein levels were analyzed by quantitative real-time-polymerase chain reaction and western blotting, respectively, in testes collected from rats at postnatal day (PND) 7, 14, 21, 35, and 90, and from rats treated with LH, 7α-methyl-19-nortestosterone (MENT) and testosterone at PND 21 and PND 90. Immunohistochemical staining was used to identify the localization of the 11β-HSD in rat testis at PND 7, 14, and 90. We found that 11β-HSD1 expression was restricted to the interstitial areas, and that its levels increased during rat testis development. In contrast, whereas 11β-HSD2 was expressed in both the interstitial areas and seminiferous tubules at PND 7, it was present only in the interstitial areas at PND 90, and its levels declined during testicular development. Moreover, 11β-HSD1 mRNA was induced by LH in both the PND 21 and 90 testes and by MENT at PND 21, whereas 11β-HSD2 mRNA was induced by testosterone and MENT in the PND 21 testis and by LH in the PND 90 testis. In conclusion, our study indicates that the 11β-HSD1 and 11β-HSD2 genes have distinct patterns of spatiotemporal expression and hormonal regulation during postnatal development of the rat testis.