Negative regulation of early B lymphopoiesis by interleukin 3 and interleukin 1 alpha.
Negative regulation of early B lymphopoiesis by interleukin 3 and interleukin 1 alpha.
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白细胞介素 3 和白细胞介素 1 α 对早期 B 淋巴细胞生成的负调节。
DOI：
10.1073/pnas.91.2.469
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发表时间：
1994
影响因子：
11.1
通讯作者：
Ogawa,M
中科院分区：
文献类型：
--
作者：
Hirayama,F;Clark,SC;Ogawa,M
我们最近开发了一个两步甲基纤维素培养系统的小鼠淋巴造血祖细胞，能够分化沿着骨髓和B淋巴谱系。在这个系统中，双因子组合，其中包括钢因子加白细胞介素（IL）6，IL-11，或粒细胞集落刺激因子有效地支持淋巴骨髓样原代集落的潜力。有趣的是，IL-3既不能取代也不能协同作用，以维持原代集落的B淋巴潜能的钢因子，虽然集落形成的频率是相同的IL-3和钢因子。我们现在报道，IL-3或IL-1 α加入到一个允许的系统中抑制了原代培养中原始祖细胞的B淋巴潜能，并呈剂量依赖性。将原代集落体内转移至scid小鼠证实了IL-3和IL-1 α的抑制作用。此外，IL-1 α抑制了二次培养物中前B细胞集落的形成。一旦前B细胞集落在二次培养中形成，两个因素都不影响前B细胞的增殖。这些结果表明，IL-3和IL-1 α在B淋巴细胞生成的早期阶段的负调节作用。
We recently developed a two-step methyl cellulose culture system for murine lymphohemopoietic progenitors that are capable of differentiation along the myeloid and B-lymphoid lineages. In this system, two-factor combinations, which include steel factor plus interleukin (IL) 6, IL-11, or granulocyte colony-stimulating factor effectively supported the lymphomyeloid potential of primary colonies. Interestingly, IL-3 could neither replace nor act synergistically with steel factor in maintaining the B-lymphoid potential of the primary colonies although the frequency of colony formation was the same with IL-3 and steel factor. We now report that addition of IL-3 or IL-1 alpha to a permissive system suppresses the B-lymphoid potential of primitive progenitor cells in primary culture in dose-dependent fashion. In vivo transfer of the primary colonies to scid mice confirmed the suppressive effects of IL-3 and IL-1 alpha. In addition, IL-1 alpha inhibited pre-B-cell colony formation in the secondary culture. Once pre-B-cell colonies had formed in secondary culture, neither factor affected the proliferation of the pre-B cells. These results suggest negative regulatory roles for IL-3 and IL-1 alpha in early stages of B lymphopoiesis.