Thermal injury-associated immunosuppression: occurrence and in vitro blocking effect of post recovery serum.
Thermal injury-associated immunosuppression: occurrence and in vitro blocking effect of post recovery serum.
复制标题
热损伤相关的免疫抑制：恢复后血清的发生和体外阻断作用。
作者：
J. Ninnemann;J. Fisher;T. Wachtel
72例烧伤病人中有38例的血清对PHA诱导的正常人淋巴细胞体外培养有明显的抑制作用。在许多这些患者中，我们观察到抑制水平随着恢复而下降。在对其中8例患者的研究中，我们发现，向正常淋巴细胞培养物中加入恢复后血清可阻断早期获得的自体血清的抑制作用。阻断似乎是通过抗体的形成实现的，因为：a）在具有阻断活性的血清样品中IgG水平大大升高，B）血清中阻断物质的出现时间与抗体的产生相容，和c）在这种回收后血清的蛋白A分离的IgG级分中含有阻断活性。
Sera from 38 of 72 burn patients have been found to be significantly suppressive to the PHA-induced blastogenesis of normal human lymphocytes in culture. In many of these patients, we have observed that suppression levels decline with recovery. In a study of eight of these patients, we have found that the addition of post recovery serum to cultures of normal lymphoyctes blocked the suppressive effect of autologous serum obtained earlier. Blocking appears to be achieved through the formation of antibodies since: a) IgG levels are greatly elevated in serum samples having blocking activity, b) the time of appearance of blocking substances in the serum is compatible with the generation of antibody, and c) blocking activity is contained in the protein-A isolated IgG fraction of such post recovery serum.