Heat-killed pneumococci and pneumococcal capsular polysaccharides stimulate tumor necrosis factor-alpha production by murine macrophages.
Heat-killed pneumococci and pneumococcal capsular polysaccharides stimulate tumor necrosis factor-alpha production by murine macrophages.
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热灭活的肺炎球菌和肺炎球菌荚膜多糖刺激小鼠巨噬细胞产生肿瘤坏死因子-α。
DOI：
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发表时间：
1994
影响因子：
6.4
通讯作者：
D. Bice
中科院分区：
文献类型：
--
作者：
S. Simpson;R. Singh;D. Bice
肿瘤坏死因子-α（TNF）是脓毒症和内毒素诱导休克的重要体液介质。然而，肺炎链球菌，一种革兰氏阳性微生物，是社区获得性肺炎和败血症的最常见病原体。我们假设，肺炎球菌性肺炎和败血症的发病机制涉及肺炎球菌刺激的TNF合成，我们测试了这一假设在体外通过比较热杀死的III型和V型肺炎球菌和23价纯化的肺炎球菌荚膜多糖与大肠杆菌和纯化的脂多糖（LPS）作为刺激TNF生产的小鼠巨噬细胞系RAW 264.7。我们评估了TNF的产生，在不同剂量和时间的暴露于这些代理商，以及吲哚美辛对TNF的生产响应这些代理商的影响。用两种类型的热灭活肺炎球菌刺激导致TNF的产生呈剂量-反应方式，用E.杆菌与V型肺炎球菌或E.但三种细菌的总体剂量-反应曲线相似。肺炎球菌荚膜多糖和LPS的剂量-反应曲线非常相似，尽管在最高浓度下，肺炎球菌荚膜多糖比LPS刺激更多的TNF分泌（469对213 U/ml）。肺炎球菌刺激的TNF分泌的动力学与LPS刺激的TNF分泌的动力学相同。在吲哚美辛的存在下，肺炎球菌刺激的TNF产生减少了87.5%，与单独的肺炎球菌相比。与此相反，LPS与吲哚美辛刺激TNF比单独LPS多19.5%。（250字处删节）
Tumor necrosis factor-alpha (TNF) is an important humoral mediator of sepsis and endotoxin-induced shock. However, Streptococcus pneumoniae, a gram-positive organism, is the most common causative agent of community-acquired pneumonia and sepsis. We hypothesized that the pathogenesis of pneumococcal pneumonia and sepsis involves pneumococcus-stimulated TNF synthesis, and we tested that hypothesis in vitro by comparing heat-killed type III and type V pneumococcus and 23-valent purified pneumococcal capsular polysaccharides with Escherichia coli and purified lipopolysaccharide (LPS) as stimuli for TNF production by the murine macrophage cell line RAW 264.7. We evaluated TNF production in response to various doses and times of exposure to these agents, as well as the effects of indomethacin on TNF production in response to these agents. Stimulation with both types of heat-killed pneumococcus resulted in TNF production in a dose-response fashion, as did stimulation with E. coli. Fewer type III pneumococci (10 bacteria/ml) were required to stimulate significant TNF secretion than either type V pneumococcus or E. coli, but the overall dose-response curves of the three bacteria were similar. The dose-response curves for pneumococcal capsular polysaccharides and LPS were very similar, although at the highest concentration pneumococcal capsular polysaccharides stimulated more TNF secretion than did LPS (469 versus 213 U/ml). The kinetics of pneumococcus-stimulated TNF secretion were identical to the kinetics of LPS-stimulated TNF secretion. In the presence of indomethacin, pneumococcus-stimulated TNF production decreased by 87.5%, as compared with pneumococcus alone. In contrast, LPS with indomethacin stimulated 19.5% more TNF than LPS alone.(ABSTRACT TRUNCATED AT 250 WORDS)
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DOI：
10.1165/ajrcmb/7.3.239
发表时间：
1992-09
影响因子：
6.4
作者：
P. Tobias;J. Mathison;Douglas N. Mintz;J. D. Lee;V. Kravchenko;K. Kato;J. Pugin;R. Ulevitch
通讯作者：
P. Tobias;J. Mathison;Douglas N. Mintz;J. D. Lee;V. Kravchenko;K. Kato;J. Pugin;R. Ulevitch
影响因子：
15.9
作者：
OKUSAWA, S;GELFAND, JA;DINARELLO, CA
通讯作者：
DINARELLO, CA
影响因子：
15.9
作者：
WAKABAYASHI, G;GELFAND, JA;DINARELLO, CA
通讯作者：
DINARELLO, CA
DOI：
--
发表时间：
1987-06
期刊：
Laboratory investigation; a journal of technical methods and pathology
影响因子：
--
作者：
Daniel G. Remick;Robin G. Kunkel;Larrick Jw;S. L. Kunkel
通讯作者：
Daniel G. Remick;Robin G. Kunkel;Larrick Jw;S. L. Kunkel
影响因子：
4.4
作者：
Michael J. Myers;Jeffrey K. Pullen;Namit Ghildyal;E. Eustis-Turf;Lawrence B. Schook
通讯作者：
Michael J. Myers;Jeffrey K. Pullen;Namit Ghildyal;E. Eustis-Turf;Lawrence B. Schook