Polymorphisms of the prolactin gene and their association with egg production traits in two Chinese domestic ducks
Polymorphisms of the prolactin gene and their association with egg production traits in two Chinese domestic ducks
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DOI：
10.1080/00071668.2019.1567909
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发表时间：
2019-03-04
影响因子：
2
通讯作者：
Li, A.
中科院分区：
文献类型：
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作者：
Bai, D. -P.;Hu, Y. -Q.;Li, A.
1.催乳素（PRL）是一种多肽激素，对产蛋性状起着至关重要的作用。2.采用DNA序列测定和聚合酶链反应-单链构象多态性（PCR-single strand conformation polymorphism，PCR-SSCP）方法，对中国2个蛋鸭品种（分别为金鼎和攸县）的PRL基因多态性进行了分析。3.结果表明，在PRL基因内含子1上检测到一个多态性位点（A-412 G），存在AA、AG和GG三种基因型。关联分析表明，GG基因型的产蛋量和蛋重显著高于AG和AA基因型（p < 0.05）。因此，PRL基因内含子1的412 A> G多态性是一个潜在的有价值的遗传标记，蛋鸭育种计划。
1. Prolactin (PRL) as a polypeptide hormone which plays a crucial role in egg production traits. 2. Polymorphisms of the PRL gene were analysed with DNA sequencing and polymerase chain reaction-single-strand conformation polymorphism methods in two Chinese domestic laying duck breeds (Jinding, n = 400, Youxian, n = 400, respectively). 3. The results showed that one polymorphism was detected (A-412G) in intron 1 of the PRL gene, with three genotypes: AA, AG and GG. Association analysis showed that the ducks with the GG genotype had significantly greater egg production and egg weight than those with AG and AA genotype (p < 0.05). Hence, the 412A > G polymorphism of the PRL gene in intron 1 is a potentially valuable genetic marker for laying duck breeding programmes.