Inflammatory response to sputum induction measured by exhaled markers
Inflammatory response to sputum induction measured by exhaled markers
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DOI：
10.1159/000086721
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发表时间：
2005-01-01
期刊：
影响因子：
3.7
通讯作者：
Barnes, PJ
中科院分区：
文献类型：
--
作者：
Antczak, A;Kharitonov, SA;Barnes, PJ
背景：痰液诱导越来越多地用于研究气道的细胞和生化组成。然而，在吸入高渗盐水后8小时，中性粒细胞的百分比显著升高。目的：本研究的目的是评估正常和哮喘受试者诱导痰后呼出气和呼出气冷凝物中的炎症标志物是否发生变化。研究方法：我们测量了白三烯B-4（LTB 4）和氧化应激的标志物8-异前列腺素，（通过酶免疫测定法）呼出空气冷凝物和呼出一氧化氮（否;化学发光分析仪测定（8例女性，平均年龄35 ± 4岁，FEV 1 97.4%预测值）和8例轻度哮喘受试者（5例男性，平均年龄34 ± 2岁，FEV 1 70.5%预测值）。结果如下：在健康受试者中，6 h时LTB 4显著高于基线，但在诱导痰液后24 h时未保持升高（基线时分别为134.3 +/- 30.15和75.4 +/- 14.32 vs. 64.6 +/- 11.6 pg/ml; p < 0.02 and p >0.05）。在健康受试者中观察到诱导痰后6 h的LTB_4与呼出气NO呈负相关（r =-0.66，p < 0.03）。在哮喘患者中未观察到LTB 4水平升高。哮喘患者的基线8-异前列烷水平高于健康受试者（47.3 +/- 37.1对17.5 +/- 8.8 pg/ ml; p < 0.01）。在健康受试者中，吸入后6和24 h可观察到8-异前列烷水平升高的趋势（分别为26.2 +/- 3.7和26.7 +/- 3.9 pg/ml; p = 0.09和p = 0.07）。在健康受试者中，呼出的NO在6小时时显著高于基线，并且在诱导痰后24小时仍保持增加（7.96 +/- 3.5 vs. 5.61 +/- 1.86 ppb;分别为p < 0.01和p < 0.05）。哮喘患者呼出的NO水平升高，但诱导痰后没有进一步升高。结论：用高渗盐水诱导痰液引起炎症反应，在使用该技术监测气道炎症时应考虑到这一点。版权所有（C）2005 S. Karger AG，巴塞尔。
Background: Sputum induction is increasingly used to study both cellular and biochemical composition of the airways. However, there is a significant rise in the percentage of neutrophils at 8 h after inhalation with hypertonic saline. Objective: The aim of this study was to assess whether markers of inflammation in exhaled air and exhaled air condensate change after sputum induction in normal and asthmatic subjects. Methods: We measured leukotriene B-4 (LTB4) and a marker of oxidative stress, 8-isoprostane, ( by enzyme immunoassay) in exhaled air condensate and exhaled nitric oxide ( NO; by chemiluminescence analyzer) in 15 healthy subjects ( 8 females, mean age 35 +/- 4 years, FEV1 97.4% predicted) and in 8 mild asthmatic subjects ( 5 males, mean age 34 +/- 2 years, FEV1 70.5% predicted). Results: LTB4 was significantly higher compared with baseline at 6 h but did not remain increased at 24 h after sputum induction (134.3 +/- 30.15 and 75.4 +/- 14.32 vs. 64.6 +/- 11.6 pg/ml at baseline; p < 0.02 and p > 0.05, respectively) in healthy subjects. An inverse correlation between LTB4 and exhaled NO at 6 h after sputum induction was observed in healthy subjects ( r = - 0.66, p < 0.03). No increase in LTB4 levels was observed in asthmatic patients. Baseline 8-isoprostane levels were higher in asthmatic patients than in healthy subjects (47.3 +/- 37.1 vs. 17.5 +/- 8.8 pg/ ml; p < 0.01). A trend towards increased levels of 8-isoprostane could be observed at 6 and 24 h after inhalation in healthy subjects (26.2 +/- 3.7 and 26.7 +/- 3.9 pg/ml; p = 0.09 and p = 0.07, respectively). In healthy subjects, exhaled NO was significantly higher compared with baseline at 6 h and remained increased 24 h after sputum induction (7.96 +/- 3.5 vs. 5.61 +/- 1.86 ppb; p < 0.01 and p < 0.05, respectively). Exhaled NO levels were increased in asthmatic patients but did not further increase after sputum induction. Conclusions: Sputum induction with hypertonic saline causes an inflammatory response which should be considered when using the technique to monitor airway inflammation. Copyright (C) 2005 S. Karger AG, Basel.