TRANSMISSIBLE GASTROENTERITIS OF SWINE - VIRUS-INTESTINAL CELL INTERACTIONS .1. IMMUNOFLUORESCENCE, HISTOPATHOLOGY AND VIRUS PRODUCTION IN SMALL INTESTINE THROUGH COURSE OF INFECTION
TRANSMISSIBLE GASTROENTERITIS OF SWINE - VIRUS-INTESTINAL CELL INTERACTIONS .1. IMMUNOFLUORESCENCE, HISTOPATHOLOGY AND VIRUS PRODUCTION IN SMALL INTESTINE THROUGH COURSE OF INFECTION
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DOI：
10.1007/bf01253767
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发表时间：
1970-01-01
期刊：
影响因子：
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通讯作者：
BURNSTEIN, T
中科院分区：
文献类型：
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作者：
PENSAERT, M;HAELTERMAN, EO;BURNSTEIN, T
用TGE病毒的Purdue株对完整猪小肠粘膜和手术分离的空肠袢中的病毒-肠细胞相互作用进行了连续研究。感染的不同阶段通过免疫荧光、组织病理学和病毒产生的测量进行检查。病毒复制仅发生在绒毛上的吸收细胞的细胞质中，并导致在PI 12小时开始的细胞变性和脱落。在空肠中，上皮细胞的损失导致，通过绒毛的进行性缩短，绒毛萎缩，在PI 16至18小时粘膜暂时剥脱。在完整猪中发生多形性细胞浸润，同时伴有大量细胞丢失。在十二指肠上段和回肠含绒毛的淋巴组织中，TGE病毒复制仅限于少数细胞，不会引起绒毛萎缩。Lieberkühn隐窝中的增殖细胞对TGE病毒不敏感，这使得病毒损伤可以通过快速替换新再生的细胞而愈合，这些细胞最初是扁平至立方形的。然后观察细胞的分化，接着是绒毛的再生长。在分化过程中，一些再生细胞被感染。然而，这些细胞中的大多数没有显示出细胞内病毒活性的任何迹象。随着绒毛的增长，感染细胞的数量逐渐减少，并在接种后7天检测不到荧光。在分离的空肠袢感染TGE病毒后发生的事件，有一些微小的变化，类似于在完整的猪。在6小时时，在分离的肠袢的管腔中首次检测到新产生的病毒。病毒产量在感染后12至24小时达到最高水平，然后在接种后15至18天逐渐下降至不可检测。
A sequential study of the virus-intestinal cell interactions in the small intestinal mucosa of intact pigs and surgically isolated loops of jejunum was made using the Purdue strain of TGE virus. The different phases of the infection were examined by immunofluorescence, histopathology and measurement of virus production. Viral replication occurred only in the cytoplasm of the absorptive cells on the villi and resulted in cellular degeneration and desquamation starting at 12 hours PI. In the jejunum, loss of the epithelium led, via progressive shortening of the villi, to villous atrophy with temporary denudation of the mucosa at 16 to 18 hours PI. Polymorphonuclear cell infiltration occurred in intact pigs concomitantly with massive cell loss. In the upper duodenum and on the villi containing lymphoid tissue in the ileum, TGE virus replication was limited to a few cells and did not cause villous atrophy.The proliferative cells in the crypts of Lieberkühn were refractory to TGE virus and this permitted healing of the viral injury to occur by rapid replacement with newly regenerated cells which initially were flat to cuboidal. Differentiation of the cells was then observed and was followed by regrowth of the villi. Some of the regenerated cells were seen to become infected during this differentiation process. The majority of these cells, however, did not show any indication of intracellular viral activity. As villi grew longer, the number of infected cells gradually decreased and fluorescence was not detected after 7 days postinoculation.The events occurring in the isolated jejunal loops upon infection with TGE virus were, with some minor variations, similar to those in intact pigs. Newly produced virus was first detected in the lumen of the isolated loops at six hours. Virus yields reached maximum levels at 12 to 24 hours after infection and then progressively declined to be non-detectable at 15 to 18 days after inoculation.