Distribution and structural diversity of cilia in tadpole larvae of the ascidian Ciona intestinalis
Distribution and structural diversity of cilia in tadpole larvae of the ascidian Ciona intestinalis
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DOI：
10.1016/j.ydbio.2009.10.012
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发表时间：
2010-01-01
影响因子：
2.7
通讯作者：
Inaba, Kazuo
中科院分区：
文献类型：
--
作者：
Konno, Alu;Kaizu, Maiko;Inaba, Kazuo
越来越多的证据表明，纤毛在胚胎发生的多种过程中发挥着重要作用。为了在细胞水平上对幼虫纤毛进行功能研究，我们利用了海鞘蝌蚪幼虫的简单细胞组织。免疫荧光显微镜显示纤毛不仅分布在先前描述的组织中，而且分布在感觉囊泡、肠道原基、乳头、顶干表皮神经元和内胚层链的室管膜细胞亚群中。透射电子显微镜揭示了多种轴丝结构。包括类似于脊椎动物初级纤毛的9+0结构、中心有电子致密材料的9+0结构、没有动力蛋白臂的9+2结构以及远端结构杂乱的轴丝。本研究中对纤毛的广泛描述为了解纤毛结构的演变提供了重要的见解，并为建立脊索动物身体计划中的纤毛功能分析提供了基础。 (C) 2009 Elsevier Inc. 保留所有权利。
Accumulating evidence demonstrates that cilia play important roles in a variety of processes in embryogenesis. For functional survey of larval cilia at the cellular level, we exploited the simple cell organization of tadpole larvae in the ascidian Ciona intestinalis. Immunofluorescent microscopy showed distribution of cilia not only in previously described tissues but also in a subpopulation of ependymal cells in the sensory vesicle, gut primordium, papillae, apical trunk epidermal neurons, and the endodermal strand. Transmission electron microscopy revealed a variety of axonemal structures. including a 9 + 0 structure similar to vertebrate primary cilia, a 9 + 0 structure with electron-dense materials in the center, a 9 + 2 structure with no dynein arms, and an axoneme with a disorganized structure at the distal end. Extensive description of cilia in the present study gives important insights into the evolution of the ciliary structure and provides a basis for analysis of ciliary functions in establishment of chordate body plan. (C) 2009 Elsevier Inc. All rights reserved.