Distinct transport mechanism in Candida albicans methylammonium permeases
Distinct transport mechanism in Candida albicans methylammonium permeases
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DOI：
10.1007/s11557-020-01625-0
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发表时间：
2020-10
影响因子：
2.4
通讯作者：
B. Neuhäuser
中科院分区：
文献类型：
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作者：
B. Neuhäuser
铵是否以 NH4+ 或 NH3 的形式跨膜运输，对于生物体的生长和发育至关重要。两种分子的跨膜运输遵循不同的 pH 依赖性梯度，并且两种底物的运输对内部和外部 pH 值有不同的影响。因此，它们直接影响生理和有机体的发育。来自白色念珠菌的 CaMep2 在铵转运和传感中显示出双重受体功能。 CaMep2 感知低铵利用率并诱导丝状生长。相比之下，CaMep1 仅在运输中活跃，但不参与铵信号传导。在这里，两种蛋白质在非洲爪蟾细胞中异源表达。这项研究确定了 CaMep1 的电生 NH4+ 转运和 CaMep2 的电中性 NH3 转运，这可能是诱导假菌丝生长的先决条件。
It is crucial for the growth and development of an organism whether ammonium is transported across its membranes in a form of NH4+or NH3. The transport of both molecules follows different pH-dependent gradients across membranes and transport of both substrates differentially affects the internal and external pH. As a consequence, they directly influence the physiology and organism development. CaMep2 fromCandida albicansshows a dual transceptor function in ammonium transport and sensing. CaMep2 senses low ammonium availability and induces filamentous growth. CaMep1, by contrast, is only active in transport, but not involved in ammonium signaling. Here, both proteins were heterologously expressed inXenopus laevisoocytes. This study identified electrogenic NH4+transport by CaMep1 and electroneutral NH3transport by CaMep2, which might be a prerequisite for the induction of pseudohyphal growth.