Systematic Identification, Evolution and Expression Analysis of the Zea mays PHT1 Gene Family Reveals Several New Members Involved in Root Colonization by Arbuscular Mycorrhizal Fungi.
Systematic Identification, Evolution and Expression Analysis of the Zea mays PHT1 Gene Family Reveals Several New Members Involved in Root Colonization by Arbuscular Mycorrhizal Fungi.
复制标题
玉米 PHT1 基因家族的系统鉴定、进化和表达分析揭示了参与丛枝菌根真菌根部定植的几个新成员
DOI：
10.3390/ijms17060930
复制
发表时间：
2016-06-13
影响因子：
5.6
通讯作者：
Cheng B
中科院分区：
文献类型：
--
作者：
Liu F;Xu Y;Jiang H;Jiang C;Du Y;Gong C;Wang W;Zhu S;Han G;Cheng B
磷酸盐转运蛋白1（PHT 1）基因家族在土壤无机磷的吸收中起着关键作用。然而，目前还没有基于全基因组对玉米中PHT 1家族进行全面研究的报道。本研究利用生物信息学的方法，在玉米自交系B73基因组中共鉴定出13个可能的PHT 1基因（ZmPHT 1;1 ~ 13）。然后，通过酵母PHO 84突变互补实验和qRT-PCR研究它们的功能。13个ZmPHT 1基因分布在6条染色体（1、2、5、7、8和10）上，可分为两个旁系同源基因（Class A和Class B）。ZmPHT 1;1/ZmPHT 1;9和ZmPHT 1;9/ZmPHT 1;13由最近的片段重复事件产生。ZmPHT 1;1/ZmPHT 1;13和ZmPHT 1;8/ZmPHT 1;10是由早期片段重复事件产生的。所有13个ZmPHT 1基因都能完全或部分地与酵母Pi摄取突变体互补，并且除了ZmPHT 1;1（p < 0.01）外，其余基因在玉米中均能被低Pi诱导表达，表明绝大多数ZmPHT 1基因都能对低Pi条件产生应答。ZmPHT1; 2、ZmPHT1; 4、ZmPHT1; 6、ZmPHT1; 7、ZmPHT1; 9和ZmPHT 1; 11个基因在AM真菌中表达上调，表明这些基因可能参与了磷的吸收和/或转运。启动子分析表明，MYCS和P1 BS元件广泛分布在不同的AMF诱导的ZmPHT 1启动子区域。根据上述结果，13个ZmPHT 1基因中有5个是新鉴定的玉米基因组中AMF诱导的高亲和力磷酸盐转运蛋白。研究结果为进一步了解PHT 1家族的进化和AMF诱导下无机磷转运的分子机制奠定了基础。
The Phosphate Transporter1 (PHT1) family of genes plays pivotal roles in the uptake of inorganic phosphate from soils. However, there is no comprehensive report on the PHT1 family in Zea mays based on the whole genome. In the present study, a total of 13 putative PHT1 genes (ZmPHT1;1 to 13) were identified in the inbred line B73 genome by bioinformatics methods. Then, their function was investigated by a yeast PHO84 mutant complementary experiment and qRT-PCR. Thirteen ZmPHT1 genes distributed on six chromosomes (1, 2, 5, 7, 8 and 10) were divided into two paralogues (Class A and Class B). ZmPHT1;1/ZmPHT1;9 and ZmPHT1;9/ZmPHT1;13 are produced from recent segmental duplication events. ZmPHT1;1/ZmPHT1;13 and ZmPHT1;8/ZmPHT1;10 are produced from early segmental duplication events. All 13 putative ZmPHT1s can completely or partly complement the yeast Pi-uptake mutant, and they were obviously induced in maize under low Pi conditions, except for ZmPHT1;1 (p < 0.01), indicating that the overwhelming majority of ZmPHT1 genes can respond to a low Pi condition. ZmPHT1;2, ZmPHT1;4, ZmPHT1;6, ZmPHT1;7, ZmPHT1;9 and ZmPHT1;11 were up-regulated by arbuscular mycorrhizal fungi (AMF), implying that these genes might participate in mediating Pi absorption and/or transport. Analysis of the promoters revealed that the MYCS and P1BS element are widely distributed on the region of different AMF-inducible ZmPHT1 promoters. In light of the above results, five of 13 ZmPHT1 genes were newly-identified AMF-inducible high-affinity phosphate transporters in the maize genome. Our results will lay a foundation for better understanding the PHT1 family evolution and the molecular mechanisms of inorganic phosphate transport under AMF inoculation.