DNA-templated cooperative formation of the luminous lanthanide complex and its analytical application to gene detection
DNA-templated cooperative formation of the luminous lanthanide complex and its analytical application to gene detection
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DOI：
10.1246/cl.2005.1606
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发表时间：
2005-12-05
影响因子：
1.6
通讯作者：
Jyo, A
中科院分区：
文献类型：
--
作者：
Kitamura, Y;Ihara, T;Jyo, A
乙二胺四乙酸 (EDTA) 和 1,10-菲咯啉 (phen) 分别共价连接到两个不同寡核苷酸 (ODN) 的 5' 和 3' 末端。 ODN缀合物的序列被设计为在目标DNA上连续杂交，其辅助单元彼此相对，提供容纳镧系离子的微环境。在完全匹配的目标 DNA 存在的情况下，可以清楚地观察到 Tb3+ 和 Eu3+ 离子的特征发射。另一方面，含有一个碱基位移的突变体的混合溶液的排放量是微乎其微的。
Ethylenediamine tetraacetic acid (EDTA) and 1,10-phenanthrorine (phen) were covalently attached to the 5' and 3' ends of two different oligonucleotides (ODNs), respectively. The sequences of the ODN conjugates were designed to contiguously hybridize on the target DNA with their auxiliary units facing each other, providing a microenvironment to accommodate the lanthanide ions. The characteristic emissions of the Tb3+ and Eu3+ ions were clearly observed in the presence of the full-matched target DNA. On the other hand, the emissions from the mixed solutions with the mutant containing one base displacement were marginal.