Clonability and tumorigenicity of human epithelial cells expressing the EBV encoded membrane protein LMP1.
Clonability and tumorigenicity of human epithelial cells expressing the EBV encoded membrane protein LMP1.
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发表时间：
1993-06
期刊：
影响因子：
8
通讯作者：
L. Hu;Fu Chen;Xi Zheng;I. Ernberg;S. Cao;B. Christensson;G. Klein;G. Winberg
中科院分区：
文献类型：
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作者：
L. Hu;Fu Chen;Xi Zheng;I. Ernberg;S. Cao;B. Christensson;G. Klein;G. Winberg
比较了EBV-LMP 1基因的两个分离株在用腺病毒12-SV 40杂交病毒永生化的非致瘤性人角质形成细胞系Rhek-1中诱导表型变化的能力。一个分离株，命名为B-LMP 1，来源于B 95 -8，一种绒猴来源的B细胞系，携带单核细胞增多症患者的病毒株。另一个命名为C-LMP 1，来源于裸鼠传代的中国NPC肿瘤，CAO。两种类型的转染子的血清依赖性比未转染和载体转染的对照。在低血清条件下生长的能力随着LMP 1表达的增加而增加。所有转染子比未转染或载体转染的对照具有更高的克隆性。软琼脂中的克隆性随着LMP 1表达的增加而增加。24个C-LMP 1转染子中的9个在SCID小鼠中产生肿瘤。其中7个侵入周围组织。12个B-LMP 1转染的Rhek-1克隆中只有1个具有致瘤性。它没有侵略性地生长。所有致瘤转染表达LMP 1在高或中等水平。所有肿瘤均表达LMP 1。低LMP 1表达的转染子不产生肿瘤。未转染的Rhek-1细胞和6个载体对照克隆不能产生肿瘤。
Two isolates of the EBV-LMP1 gene were compared for their ability to induce phenotypic changes in a non-tumorigenic human keratinocyte line, Rhek-1, immortalized with an adenovirus 12-SV40 hybrid virus. One isolate, designated B-LMP1, was derived from B95-8, a B-cell line of marmoset origin, that carries a viral strain from a mononucleosis patient. The other, designated C-LMP1, originated from a nude mouse passaged Chinese NPC tumor, CAO. Both types of transfectants were less serum dependent than the non-transfected and the vector-transfected controls. The ability to grow on low serum increased with increasing LMP1 expression. All transfectants were more highly clonable than the non-transfected or vector-transfected controls. Clonability in soft agarose increased with increasing LMP1 expression. Nine of 24 C-LMP1 transfectants produced tumors in SCID mice. Seven of them grew invasively into the surrounding tissue. Only one of 12 B-LMP1 transfected Rhek-1 clones was tumorigenic. It did not grow invasively. All tumorigenic transfectants expressed LMP1 at high or moderate levels. All tumors were found to express LMP1. Transfectants with low LMP1 expression did not produce tumors. The untransfected Rhek-1 cells and six vector control clones failed to produce tumors.