Compromised stability of DNA methylation and transposon immobilization in mosaic Arabidopsis epigenomes
Compromised stability of DNA methylation and transposon immobilization in mosaic Arabidopsis epigenomes
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DOI：
10.1101/gad.524609
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发表时间：
2009-04-15
影响因子：
10.5
通讯作者：
Paszkowski, Jerzy
中科院分区：
文献类型：
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作者：
Reinders, Jon;Wulff, Brande B. H.;Paszkowski, Jerzy
跨代表观遗传是通过对相对较少的基因座的研究来定义的。我们研究了一组重组自交系，其表观遗传马赛克染色体由野生型和CG甲基化缺失片段(EPERRIL)组成。令人惊讶的是，在亲本系中不活动的转座子在28%的外源基因中表现出随机运动。尽管在全基因组DNA甲基化图谱的支持下，经过八代近亲繁殖后的分析发现了重组的亲本染色体片段，但这些片段中夹杂着出人意料的高频率的非亲本甲基化多态。因此，来自表观基因组不同的双亲的杂交种的表观遗传允许持久的表观等位基因相互作用，这违反了孟德尔的期望。这种持续的“不稳定”表观遗传状态使基于连锁的表观基因组图谱复杂化。因此，未来的表观基因组分析应该考虑可能的遗传不稳定性和替代的作图策略。
Transgenerational epigenetic inheritance has been defined by the study of relatively few loci. We examined a population of recombinant inbred lines with epigenetically mosaic chromosomes consisting of wild-type and CG methylation-depleted segments (epiRILs). Surprisingly, transposons that were immobile in the parental lines displayed stochastic movement in 28% of the epiRILs. Although analysis after eight generations of inbreeding, supported by genome-wide DNA methylation profiling, identified recombined parental chromosomal segments, these were interspersed with unexpectedly high frequencies of nonparental methylation polymorphism. Hence, epigenetic inheritance in hybrids derived from parents with divergent epigenomes permits long-lasting epi-allelic interactions that violate Mendelian expectations. Such persistently "unstable'' epigenetic states complicate linkage-based epigenomic mapping. Thus, future epigenomic analyses should consider possible genetic instabilities and alternative mapping strategies.