Nine Novel Phages from a Plateau Lake in Southwest China: Insights into Aeromonas Phage Diversity
Nine Novel Phages from a Plateau Lake in Southwest China: Insights into Aeromonas Phage Diversity
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中国西南高原湖泊的九种新型噬菌体：深入了解气单胞菌噬菌体多样性
DOI：
10.3390/v11070615
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发表时间：
2019-07-01
期刊：
影响因子：
4.7
通讯作者：
Xiao, Wei
中科院分区：
文献类型：
--
作者：
Bai, Meng;Cheng, Ya-Hui;Xiao, Wei
气单胞菌属是鱼类常见的病原菌，其中一些可以机会性地引起人类的传染病。抗生素的过度使用导致了细菌耐药性的出现。迄今为止，只有51个完整的基因组序列在GenBank中。本文报道了从我国高原湖泊中分离到的9株嗜水气单胞菌。对所有60个目前测序的气单胞菌噬菌体基因组进行了蛋白质簇、点图和ANI分析，并将其分为9个簇和13个单个体。其中2L 372 D（包括2L 372 D、2L 372 X、4L 372 D、4L 372 XY）的DNA包装策略尚不清楚，而其它5个基因簇的DNA包装策略均为头部（P22/Sf6）。值得注意的是，具有较大基因组的分离噬菌体保守地编码辅助代谢基因、DNA复制和代谢基因，而在较小的噬菌体基因组中，重组相关基因是保守的。最后，我们提出了一个新的分类方案的气单胞菌。
Aeromonas species are common pathogens of fish and some of them can opportunistically cause infectious diseases in humans. The overuse of antibiotics has led to the emergence of bacterial drug-resistance. To date, only 51 complete genome sequences of Aeromonas phages are available in GenBank. Here, we report the isolation of nine Aeromonas phages from a plateau lake in China. The protein cluster, dot plot and ANI analyses were performed on all 60 currently sequenced Aeromonas phage genomes and classified into nine clusters and thirteen singletons. Among the nine isolated phages, the DNA-packaging strategy of cluster 2L372D (including 2L372D, 2L372X, 4L372D, 4L372XY) is unknown, while the other five phages use the headful (P22/Sf6) DNA-packaging strategy. Notably, the isolated phages with larger genomes conservatively encode auxiliary metabolism genes, DNA replication and metabolism genes, while in smaller phage genomes, recombination-related genes were conserved. Finally, we propose a new classification scheme for Aeromonas phages.