Self-induced splitting of spiral-shaped spreading depression waves in chicken retina
Self-induced splitting of spiral-shaped spreading depression waves in chicken retina
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DOI：
10.1007/pl00005700
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发表时间：
1997-06-01
影响因子：
2
通讯作者：
Muller, SC
中科院分区：
文献类型：
--
作者：
Dahlem, MA;Muller, SC
脑电活动的扩散性抑制(SD)是中枢神经系统(CNS)的一种动态波动现象。视网膜，特别是分离的鸡视网膜，是观察SD波前动态行为的中枢神经系统的极好组成部分，因为它在SD攻击期间改变了它的光学性质。在黑色的背景上，海浪变成了乳白色的锋面。SD的基本机制步骤是什么仍存在争议，但可以肯定的是，SD属于神经元组织中的自组织现象。在这项工作中，螺旋波前分析使用数字视频成像技术。我们报道了波前的内端，也就是螺旋尖端是如何反复脱离的。该分离过程与Z形轨迹(延伸类似于1.2 mm)有关，该轨迹由尖端在一次螺旋旋转(周期2.45+/-0.1分钟)上描述。Z形轨迹并不是固定不变的，而是在每个时期都会在视网膜上进行一次复杂的运动。据我们所知，这是第一次将已建立的成像方法应用于研究SD波传播的二维特征并获得其动力学的定量数据。由于这些方法不干扰组织，因此可以在不受任何外部影响的情况下观察该现象的内在属性。
Spreading depression (SD) of electroencephalographic activity is a dynamic wave phenomenon in the central nervous system (CNS). The retina, especially the isolated chicken retina, is an excellent constituent of the CNS in which to observe the dynamic behavior of the SD wave fronts, because it changes its optical properties during a SD attack. The waves become visible as milky fronts on a black background. It is still controversial what the basic mechanistic steps of SD are, but certainly SD belongs to the self-organization phenomena occurring in neuronal tissue. In this work, spiral-shaped wave fronts are analyzed using digital video imaging techniques. We report how the inner end of the wave front, the spiral tip, breaks away repeatedly. This separation process is associated with a Z-shaped trajectory (extension similar to 1.2 mm) that is described by the tip over one spiral revolution (period 2.45+/-0.1 min). The Z-shaped trajectory does not remain fixed, but performs a complex motion across the retina with each period. This is the first time, to our knowledge, that established imaging methods have been applied to the study of the two-dimensional features of SD wave propagation and to obtaining quantitative data of their dynamics. Since these methods do not interfere with the tissue, it is possible to observe the intrinsic properties of the phenomenon without any external influence.