Phylogenetic analysis and characterization of bovine herpesvirus-1 in cattle of China, 2016-2019.
Phylogenetic analysis and characterization of bovine herpesvirus-1 in cattle of China, 2016-2019.
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DOI：
10.1016/j.meegid.2020.104416
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发表时间：
2020-06
期刊：
影响因子：
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通讯作者：
Yulong Zhou;Xuyang Li;Yachao Ren;X. Hou;Yu Liu;Sumin Wei;Guanli Dai;Ye Meng;Linjie Hu;Zhihui Liu;W. Jia;Zhanbo Zhu;Rui Wu
中科院分区：
文献类型：
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作者：
Yulong Zhou;Xuyang Li;Yachao Ren;X. Hou;Yu Liu;Sumin Wei;Guanli Dai;Ye Meng;Linjie Hu;Zhihui Liu;W. Jia;Zhanbo Zhu;Rui Wu
牛疱疹病毒1型（BoHV-1）是牛中最重要的病原体之一，在中国流行。BoHV-1分为BoHV-1.1和bohv -1.2两种基因型，并进一步分化为BoHV-1.2a和1.2b两种亚型。然而，BoHV-1分离株的系统发育分析在中国尚未见报道。为了开展基于中国牛分离物的分子流行病学调查，利用2016年至2019年在中国死亡的102头10月龄以下呼吸道疾病（BRD）犊牛肺组织样本，用Madin-Darby牛肾（MDBK）细胞分离BoHV-1。利用部分BoHV-1分离株的糖蛋白C （gC）基因区域进行系统发育分析。获得30株BoHV-1分离株，采用聚合酶链反应（PCR）方法扩增其中13株的gC基因并测序。根据gC基因451个核苷酸部分的系统发育分析结果，13株分离株均属于BoHV-1.2b基因亚型，但这些分离株位于两个不同的系统发育树枝上。组1分离株gC基因序列同源性较高，与bohv - 12 b EVI14为98.0 ~ 100%，组2分离株gC基因序列同源性较高，与bohv - 12 b B589为97.8 ~ 99.8%。第2组分离物gC氨基酸序列与干扰菌株bohv - 12 b K22或BoHV-1.1 COOPER有两个氨基酸突变。2组BoHV-1分离株的细胞病变效应（cpe）在MDBK细胞上呈火山状溃散，与传统BoHV-1分离株的cpe不同。总体而言，bohv - 12 b似乎是中国牛BoHV-1的主要毒株，也是BRD的一个关键原因。这些bohv - 12 b分离株具有显著的遗传变异。
Bovine herpesvirus type 1 (BoHV-1) is one of the most critical pathogens in cattle and is prevalent in China. BoHV-1 is divided into two gene types, BoHV-1.1 and 1.2, which are further differentiated into two subtypes, BoHV-1.2a and 1.2b. However, the phylogenetic analysis of BoHV-1 isolates has not been reported in China. To perform a molecular epidemiological survey based on isolates from cattle in China, 102 lung tissue samples of calves under ten months of age with respiratory disease (BRD) that died from 2016 to 2019 in China were used to isolate BoHV-1 with Madin-Darby bovine kidney (MDBK) cells. Part of the BoHV-1 isolates were applied to the phylogenetic analysis based on the region of the glycoprotein C (gC) gene of BoHV-1. Thirty BoHV-1 isolates were obtained, and the gC gene of 13 isolates was amplified by polymerase chain reaction (PCR) methods and sequenced. The result of the phylogenetic analysis according to the 451-nucleotide portion of the gC gene found that all of 13 isolates belonged to the BoHV-1.2b gene subtype, but these isolates had located two different phylogenetic tree branches. The gC gene sequence homology of isolates in group1 was higher with a reference strain of BoHV-1.2b EVI14 up to 98.0–100%, while in group 2, this was higher with reference strain BoHV-1.2b B589 up to 97.8–99.8%. The deduced amino acid sequence of gC from isolates in group 2 had two amino acid mutations with interference strain BoHV-1.2b K22 or BoHV-1.1 COOPER. The cytopathic effects (CPEs) of BoHV-1 isolates in group 2 were ulcered on the centration like a volcano on MDBK cell, and different from traditional CPEs of BoHV-1. Overall, BoHV-1.2b seems to be the primary strain of BoHV-1 in cattle in China and is also a critical cause of BRD. These BoHV-1.2b isolates had significant genetic variations.