B7-h1 expression on non-small cell lung cancer cells and its relationship with tumor-infiltrating lymphocytes and their PD-1 expression
B7-h1 expression on non-small cell lung cancer cells and its relationship with tumor-infiltrating lymphocytes and their PD-1 expression
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B7 - h1在非小细胞肺癌细胞上的表达及其与肿瘤浸润淋巴细胞及其程序性死亡受体1（PD - 1）表达的关系
DOI：
10.1158/1078-0432.ccr-04-0428
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发表时间：
2004-08-01
影响因子：
11.5
通讯作者：
Nishimura, M
中科院分区：
文献类型：
--
作者：
Konishi, J;Yamazaki, K;Nishimura, M
目的：B7-H1/PD-L1(B7-H1)和B7-DC/PD-L2(B7-DC)是PD-1受体的配体。本研究探讨B7-H1和B7-DC在非小细胞肺癌组织中的表达及其与临床病理参数和术后生存期的关系。实验设计：采用免疫组织化学方法检测52例非小细胞肺癌手术切除标本中B7-H1和B7-DC的表达。结果：所有非小细胞肺癌标本均有B7-H1和B7-DC的局灶性表达。B7-H1和B7-DC的表达与临床病理参数和术后生存无关。然而，在相同的评估切片中，在5例患者中，B7-H1阳性肿瘤区域的TIL明显少于B7-H1阴性肿瘤区域的TIL(P=0.01)。此外，B7-H1阳性肿瘤区域表达PD-1的TIL百分率明显低于B7-H1阴性肿瘤区域(P=0.02)。结论：B7-H1在局部肿瘤细胞上的表达与TIL数量呈负相关，这可能是非小细胞肺癌抗肿瘤免疫反应的负性调节机制之一。
Purpose: B7-H1/PD-L1 (B7-H1) and B7-DC/PD-L2 (B7-DC) are ligands for the receptor PD-1, which is known to negatively regulate T-cell activation. In the present study, we investigated the expression of B7-H1 and B7-DC in tumor specimens of non-small cell lung cancer and their relationships with clinicopathological variables and postoperative survival. Furthermore, we examined the correlation between B7-H1 expression on tumor cells and the number of tumor-infiltrating lymphocytes (TILs) or PD-1 expression on TILs.Experimental Design: The expression of B7-H1 and B7-DC in 52 surgically resected specimens of non-small cell lung cancer was evaluated immunohistochemically.Results: Expression of B7-H1 and B7-DC was focally observed in all non-small cell lung cancer tumor specimens. No relationship was found between the expression of B7-H1 or B7-DC and clinicopathological variables or postoperative survival. However, in the same sections evaluated, significantly fewer TILs were identified in B7-H1-positive tumor regions than in B7-H1-negative tumor regions in a subset of five patients (P = 0.01). Moreover, the percentage of TILs expressing PD-1 was significantly lower in B7-H1-positive tumor regions than in B7-H1-negative tumor regions (P = 0.02).Conclusions: The expression of B7-H1 on tumor cells in local areas reciprocally correlated with the number of TILs, and this may contribute to negative regulation in antitumor immune responses in non-small cell lung cancer.