Somatic mitochondrial DNA mutations in prostate cancer and normal appearing adjacent glands in comparison to age-matched prostate samples without malignant histology
Somatic mitochondrial DNA mutations in prostate cancer and normal appearing adjacent glands in comparison to age-matched prostate samples without malignant histology
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DOI：
10.2353/jmoldx.2006.050112
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发表时间：
2006-07-01
影响因子：
4.1
通讯作者：
Thayer, Robert E.
中科院分区：
文献类型：
--
作者：
Parr, Ryan L.;Dakubo, Gabriel D.;Thayer, Robert E.
体细胞线粒体 DNA 突变的研究已成为癌症研究的一个重要方面，因为这些突变可能具有功能意义和/或作为肿瘤检测的生物传感器。在这里，我们报告了从 24 个前列腺切除样本中回收的三种特定组织类型（肿瘤、邻近良性和远处良性）的体细胞线粒体 DNA 突变。来自 12 名接受前列腺活检但组织学良性（症状良性）的个体的针吸活检组织被用作个体对照样本。我们还从每位患者身上采集了血液（种质组织）样本，作为与采样的体细胞组织（恶性、邻近和远处良性）相关的个体对照。尝试对每个样本进行完整的线粒体基因组测序。与两个对照组[患者（血液）和患者（有症状的良性）]相比，从恶性组恢复的所有组织类型都具有显着不同的线粒体DNA（mtDNA）突变。我们得出的结论是，线粒体基因组突变是前列腺组织恶性转化的早期指标。这些突变发生在组织病理学变化（表明前列腺癌）对病理学家来说是明显的之前。
Studies of somatic mitochondrial DNA mutations have become an important aspect of cancer research because these mutations might have functional significance and/or serve as a biosensor for tumor detection. Here we report somatic mitochondrial DNA mutations from three specific tissue types (tumor, adjacent benign, and distant benign) recovered from 24 prostatectomy samples. Needle biopsy tissue from 12 individuals referred for prostate biopsy, yet histologically benign (symptomatic benign), were used as among individual control samples. We also sampled blood (germplasm tissue) from each patient to serve as within individual controls relative to the somatic tissues sampled (malignant, adjacent, and distant benign). Complete mitochondrial genome sequencing was attempted on each sample. in contrast to both control groups [within patient (blood) and among patient (symptomatic benign)], all of the tissue types recovered from the malignant group harbored significantly different mitochondrial DNA (mtDNA) mutations. We conclude that mitochondrial genome mutations are an early indicator of malignant transformation in prostate tissue. These mutations occur well before changes in tissue histopathology, indicative of prostate cancer, are evident to the pathologist.