PROTECTION FROM GENITAL HERPES-SIMPLEX VIRUS TYPE-2 INFECTION BY VACCINATION WITH CLONED TYPE-1 GLYCOPROTEIN-D
PROTECTION FROM GENITAL HERPES-SIMPLEX VIRUS TYPE-2 INFECTION BY VACCINATION WITH CLONED TYPE-1 GLYCOPROTEIN-D
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DOI：
10.1126/science.2983428
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发表时间：
1985-01-01
期刊：
影响因子：
56.9
通讯作者：
LASKY, LA
中科院分区：
文献类型：
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作者：
BERMAN, PW;GREGORY, T;LASKY, LA
用基因工程哺乳动物细胞系gD10.2中产生的截短型单纯疱疹病毒1型（HSV-1）糖蛋白D接种豚鼠。接种疫苗的动物在体外中和试验中形成中和HSV-1和单纯疱疹病毒2型（HSV-2）的抗体。通过阴道内感染用HSV-2攻击接种动物。接受弗氏完全佐剂中的免疫原的动物完全免于生殖器HSV-2感染的临床表现。接受免疫原掺入明矾佐剂的动物部分免受临床疾病的影响;确实发生的感染比单独注射佐剂的对照动物严重得多。因此，用纯化的病毒蛋白免疫可以在豚鼠中提供针对HSV-2的原发性生殖器感染的显著保护。
Guinea pigs were vaccinated with truncated herpes simplex virus type-1 (HSV-1) glycoprotein D produced in the genetically engineered mammalian cell line gD10.2. Vaccinated animals formed antibodies that neutralized both HSV-1 and herpes simplex virus type 2 (HSV-2) in an in vitro neutralization assay. Vaccinated animals were challenged with HSV-2 by intravaginal infection. Animals that received the immunogen in Freund''s complete adjuvant were completely protected from the clinical manifestations of genital HSV-2 infection. Animals that received the immunogen incorporated in alum adjuvants were partly protected from clinical disease; the infections that did develop were significantly less severe than those that occurred in control animals injected with adjuvant alone. Thus, immunization with a purified viral protein can provide significant protection against primary genital infection by HSV-2 in guinea pigs.