Fucose-containing glycolipids are stage- and region-specific antigens in developing embryonic brain of rodents.
Fucose-containing glycolipids are stage- and region-specific antigens in developing embryonic brain of rodents.
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含岩藻糖的糖脂是啮齿类动物胚胎脑发育过程中的阶段和区域特异性抗原。
DOI：
10.1073/pnas.82.9.3045
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发表时间：
1985
影响因子：
11.1
通讯作者：
Schwarting,GA
中科院分区：
文献类型：
--
作者：
Yamamoto,M;Boyer,AM;Schwarting,GA
一种针对胚胎大鼠前脑匀浆的单抗(针对7A抗原)，标志着哺乳动物中枢神经系统中区域和发育阶段特异的细胞表面抗原的分布。在小鼠中，免疫细胞化学染色显示，7A抗原几乎只在大脑皮层的生发层表达，早在怀孕第11天就开始表达，出生时就检测不到了。典型的染色出现在胚胎第13-15天，只有大脑皮层的脑室和脑室下区被强烈标记，而大脑的大多数其他区域没有标记。组织培养研究表明，7A抗原定位于质膜。具有该抗原的分子物种被鉴定为含有X决定簇三糖的中性糖脂：(公式；见正文)这种糖结合物在许多哺乳动物物种(小鼠、大鼠、兔子、小牛和人类)的胚胎脑中表达，可能是中枢神经系统正常发育过程中重要的细胞表面成分。
A monoclonal antibody (against 7A antigen), raised against a homogenate of embryonic rat forebrain, marks the distribution of region- and developmental stage-specific cell-surface antigens in the mammalian central nervous system. In the mouse, immunocytochemical staining revealed that 7A antigen is expressed almost exclusively in germinal layers of the cerebral cortex beginning as early as day 11 of gestation and becomes undetectable by birth. The typical staining is seen at embryonic days 13-15, in which only the ventricular and the subventricular zones of the cerebral cortex are intensely labeled, whereas most other areas of the brain are unlabeled. Tissue culture studies indicate that the 7A antigen is localized at the plasma membrane. Molecular species possessing the antigen are identified as neutral glycolipids that contain the X-determinant trisaccharide: (formula; see text) This glycoconjugate is expressed in embryonic brains of many mammalian species (mouse, rat, rabbit, calf, and human) and may be a cell-surface component important in normal development of the central nervous system.