Direct Ethanol Production from Xylan and Acorn Using the Starch-Fermenting Basidiomycete Fungus Phlebia acerina
Direct Ethanol Production from Xylan and Acorn Using the Starch-Fermenting Basidiomycete Fungus Phlebia acerina
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DOI：
10.3390/fermentation7030116
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发表时间：
2021-07
期刊：
影响因子：
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通讯作者：
K. Okamoto;Takato Goda;T. Yamada;Masafumi Nagoshi
中科院分区：
文献类型：
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作者：
K. Okamoto;Takato Goda;T. Yamada;Masafumi Nagoshi
在我们寻找广泛底物生产乙醇的担子菌真菌的过程中，我们分离到了白腐担子菌Phlebia acerina，这是一种白腐担子菌。它能很好地将淀粉转化为乙醇，产率约为70%。虽然产量随着淀粉浓度的增加而下降，但即使在200 g/L的淀粉浓度下也能观察到生长和发酵。P. acerina从葡萄糖、半乳糖、甘露糖、木糖、纤维素二糖和麦芽糖中生产乙醇，产率分别为93%、91%、86%、72%、92%和68%。此外，分泌木聚糖酶和木糖苷酶的针叶假单胞菌能够吸收木聚糖并将其直接转化为乙醇，产量为63%。此外，P. acerina直接从含有淀粉和单宁的植物果实橡子中生产乙醇，产率为70%。单宁延缓菌丝生长，从而延长乙醇产量；然而，这并没有特别影响产量。这些结果与目标作物橡子在含有相同数量淀粉和单宁的培养基中发酵的结果相似，因此表明P. acerina可以成功地从含淀粉的木质纤维素生物质中生产环境友好型乙醇，而不像以前报道的生产乙醇的担子菌真菌。
During our search for ethanol-producing basidiomycete fungi for a wide range of substrates, we isolated Phlebia acerina, which is a white rot basidiomycete fungus. It favorably converted starch into ethanol with approximately 70% yield. Although the yield decreased as the starch concentration increased, growth and fermentation were observed even at 200 g/L of starch. P. acerina produced ethanol from glucose, galactose, mannose, xylose, cellobiose, and maltose with 93%, 91%, 86%, 72%, 92%, and 68% yields, respectively. Additionally, P. acerina, which secreted xylanase and xylosidase, was capable of assimilating xylan and directly converting it to ethanol with a yield of 63%. Furthermore, P. acerina produced ethanol directly from acorns, which are plant fruits containing starch and tannins, with a yield of 70%. Tannin delayed mycelia growth, thus prolonging ethanol production; however, this did not particularly affect the yield. These results were similar to those of fermentation in a medium with the same amounts of starch and tannin as the target crop acorn, thus suggesting that P. acerina could successfully produce environmentally friendly ethanol from starch-containing lignocellulosic biomass, unlike previously reported ethanol-producing basidiomycete fungi.