Control over the morphology and segregation of Zebrafish germ cell granules during embryonic development.
Control over the morphology and segregation of Zebrafish germ cell granules during embryonic development.
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DOI：
10.1186/1471-213x-8-58
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发表时间：
2008-05-28
影响因子：
--
通讯作者：
Raz E
中科院分区：
文献类型：
--
作者：
Strasser MJ;Mackenzie NC;Dumstrei K;Nakkrasae LI;Stebler J;Raz E
斑马鱼生殖细胞包含颗粒状结构，围绕细胞核排列。这些结构与果蝇的极粒、非洲爪蟾的生殖颗粒以及小鼠生殖细胞的拟染色体等具有共同特征，例如斑马鱼的Vasa、Piwi和Nanos等蛋白质的定位。在早期生殖细胞发育过程中，对于这些颗粒的结构以及它们在有丝分裂中的分离情况知之甚少。
利用表达一种被称为Granulito的荧光标记的斑马鱼生殖细胞颗粒新成分的转基因鱼，我们追踪了这些颗粒的形态和分布。我们发现，虽然这些颗粒最初在大小上有很大差异，但在发育的第一天结束时，它们变成了大小均一的中等颗粒群体。我们研究了这种大小调整事件，并证明了微管以及依赖微管负端的动力蛋白动力蛋白（Dynein）在这一过程中的作用。最后，我们表明生殖细胞颗粒驻留蛋白——含都铎结构域蛋白 - 7（Tdrd7）的功能对于颗粒形态和数量的确定是必需的。
我们的研究结果表明，斑马鱼生殖细胞颗粒经历一个转变过程，这一过程涉及生殖细胞特异性蛋白质以及微管网络。
Zebrafish germ cells contain granular-like structures, organized around the cell nucleus. These structures share common features with polar granules in Drosophila, germinal granules in Xenopus and chromatoid bodies in mice germ cells, such as the localization of the zebrafish Vasa, Piwi and Nanos proteins, among others. Little is known about the structure of these granules as well as their segregation in mitosis during early germ-cell development. Using transgenic fish expressing a fluorescently labeled novel component of Zebrafish germ cell granules termed Granulito, we followed the morphology and distribution of the granules. We show that whereas these granules initially exhibit a wide size variation, by the end of the first day of development they become a homogeneous population of medium size granules. We investigated this resizing event and demonstrated the role of microtubules and the minus-end microtubule dependent motor protein Dynein in the process. Last, we show that the function of the germ cell granule resident protein the Tudor domain containing protein-7 (Tdrd7) is required for determination of granule morphology and number. Our results suggest that Zebrafish germ cell granules undergo a transformation process, which involves germ cell specific proteins as well as the microtubular network.