Two-dimensional fluorescence difference gel electrophoretic analysis of Streptococcus mutans biofilms
Two-dimensional fluorescence difference gel electrophoretic analysis of Streptococcus mutans biofilms
复制标题
DOI：
10.1021/pr0502471
复制
发表时间：
2005-11-01
影响因子：
4.4
通讯作者：
Jacques, NA
中科院分区：
文献类型：
--
作者：
Rathsam, C;Eaton, RE;Jacques, NA
与传统的二维（2D）蛋白质组分析相比，变形链球菌作为生物膜从稳态的增殖培养物中生长（Rathsam等人，Microbiol. 2005，151，18231837），使用2D荧光差异凝胶电泳（DIGE）导致鉴定的蛋白质点的数量增加3倍，所述蛋白质点的表达水平显著改变（P < 0.050）。在73种鉴定的蛋白质中，只有9种在生物膜生长的细胞中上调。这些结果支持了先前推测的假设，即在成熟的S.变形杆菌生物膜证实了感受态蛋白的上调，而没有任何伴随的应激反应蛋白的增加，同时参与葡聚糖形成的葡糖基转移酶C（GtfC）、参与[Fe-S]簇形成的O-乙酰丝氨酸磺酰化酶（半胱氨酸合成酶A; CsyK）和由开放阅读框编码的假定蛋白SMu 0188的水平也被上调。
Compared with traditional two-dimensional (2D) proteome analysis of Streptococcus mutans grown as a biofilm from a planktonic culture at steady state (Rathsam et al., Microbiol. 2005, 151, 18231837), the use of 2D fluorescence difference gel electrophoresis (DIGE) led to a 3-fold increase in the number of identified protein spots that were significantly altered in their level of expression (P < 0.050). Of the 73 identified proteins, only nine were up-regulated in biofilm grown cells. The results supported the previously surmised hypothesis that general metabolic functions were down-regulated in response to a reduction in growth rate in mature S. mutans biofilms. Up-regulation of competence proteins without any concomitant increase in stress-responsive proteins was confirmed, while the levels of glucosyltransferase C (GtfC), involved in glucan formation, O-acetylserine sulfhyrylase (cysteine synthetase A; CsyK), implicated in the formation of [Fe-S] clusters, and a hypothetical protein encoded by the open reading frame, SMu0188, were also up-regulated.