A computational model for the formation of lamin-B mitotic spindle envelope and matrix.
A computational model for the formation of lamin-B mitotic spindle envelope and matrix.
复制标题
核纤层蛋白-B 有丝分裂纺锤体包膜和基质形成的计算模型。
DOI：
10.1098/rsfs.2013.0063
复制
发表时间：
2014
期刊：
影响因子：
4.4
通讯作者：
Iglesias,PabloA
中科院分区：
文献类型：
--
作者：
Shi,Changji;Channels,WilburE;Zheng,Yixian;Iglesias,PabloA
最近的报道表明，在核膜破裂后，核纤层的一个组成部分，lamin-B，在间期，定位于周围的有丝分裂纺锤体作为一个膜网络。然而，这一过程如何发生，以及它如何影响有丝分裂纺锤体形态发生尚不清楚。在这里，我们开发了一个计算模型的基础上的连续描述，以代表有丝分裂过程中所涉及的各种分子物种的丰度和位置，并使用该模型来测试一些假设的有丝分裂基质的形成。我们的模型表明，自由扩散的核蛋白可以被捕获和运输到纺锤体极负端定向微管（MT）电机。此外，模拟表明，这些蛋白质可用于建立一个壳状区域，信封的有丝分裂纺锤体，这有助于提高有丝分裂纺锤体的聚焦空间限制MT聚合和限制自由MT的有效扩散。模拟还证实，通过Ran系统对纺锤体网络的空间依赖性调节改善了纺锤体聚焦和形态。我们的研究结果同意实验观察，层粘连蛋白B重组周围的纺锤体，并有助于维持纺锤体形态。
Recent reports show that, after nuclear envelope breakdown, lamin-B, a component of the nuclear lamina in interphase, localizes around the mitotic spindle as a membranous network. How this process occurs, however, and how it influences mitotic spindle morphogenesis is unclear. Here, we develop a computational model based on a continuum description to represent the abundance and location of various molecular species involved during mitosis, and use the model to test a number of hypotheses regarding the formation of the mitotic matrix. Our model illustrates that freely diffusible nuclear proteins can be captured and transported to the spindle poles by minus-end-directed microtubule (MT) motors. Moreover, simulations show that these proteins can be used to build a shell-like region that envelopes the mitotic spindle, which helps to improve the focusing of the mitotic spindle by spatially restricting MT polymerization and limiting the effective diffusion of the free MTs. Simulations also confirm that spatially dependent regulation of the spindle network through the Ran system improves spindle focusing and morphology. Our results agree with experimental observations that lamin-B reorganizes around the spindle and helps to maintain spindle morphology.